Assessing the Biocompatibility and Regeneration of Electrospun-Nanofiber Composite Tracheal Grafts.
Tracheal replacement
animal model
biocompatible splint
regenerative medicine
Journal
The Laryngoscope
ISSN: 1531-4995
Titre abrégé: Laryngoscope
Pays: United States
ID NLM: 8607378
Informations de publication
Date de publication:
14 Aug 2023
14 Aug 2023
Historique:
revised:
25
07
2023
received:
07
02
2023
accepted:
26
07
2023
medline:
14
8
2023
pubmed:
14
8
2023
entrez:
14
8
2023
Statut:
aheadofprint
Résumé
Composite tracheal grafts (CTG) combining decellularized scaffolds with external biomaterial support have been shown to support host-derived neotissue formation. In this study, we examine the biocompatibility, graft epithelialization, vascularization, and patency of three prototype CTG using a mouse microsurgical model. Tracheal replacement, regenerative medicine, biocompatible airway splints, animal model. CTG electrospun splints made by combining partially decellularized tracheal grafts (PDTG) with polyglycolic acid (PGA), poly(lactide-co-ε-caprolactone) (PLCL), or PLCL/PGA were orthotopically implanted in mice (N = 10/group). Tracheas were explanted two weeks post-implantation. Micro-Computed Tomography was conducted to assess for graft patency, and histological analysis was used to assess for epithelialization and neovascularization. Most animals (greater than 80%) survived until the planned endpoint and did not exhibit respiratory symptoms. MicroCT confirmed the preservation of graft patency. Grossly, the PDTG component of CTG remained intact. Examining the electrospun component of CTG, PGA degraded significantly, while PLCL+PDTG and PLCL/PGA + PDTG maintained their structure. Microvasculature was observed across the surface of CTG and infiltrating the pores. There were no signs of excessive cellular infiltration or encapsulation. Graft microvasculature and epithelium appear similar in all groups, suggesting that CTG did not hinder endothelialization and epithelialization. We found that all electrospun nanofiber CTGs are biocompatible and did not affect graft patency, endothelialization and epithelialization. Future directions will explore methods to accelerate graft regeneration of CTG. N/A Laryngoscope, 2023.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : NIH NHLBI K08HL138460
Pays : United States
Organisme : NIH HHS
ID : NIH NHLBI R01HL157039
Pays : United States
Informations de copyright
© 2023 The American Laryngological, Rhinological and Otological Society, Inc.
Références
Grillo HC. Surgery of the Trachea and Bronchi. Lewiston, NY: BC Decker; 2004.
Delaere P, Vranckx J, Verleden G, de Leyn P, van Raemdonck D. Tracheal allotransplantation after withdrawal of immunosuppressive therapy. N Engl J Med. 2010;362:138-145.
Liu L, Dharmadhikari S, Shontz KM, et al. Regeneration of partially decellularized tracheal scaffolds in a mouse model of orthotopic tracheal replacement. J Tissue Eng. 2021;12:12. https://doi.org/10.1177/20417314211017417.
Liu L, Dharmadhikari S, Spector BM, et al. Tissue-engineered composite tracheal grafts create mechanically stable and biocompatible airway replacements. J Tissue Eng. 2022;13:20417314221108791. https://doi.org/10.1177/20417314221108791 PMID: 35782992; PMCID: PMC9243572.
Tan ZH, Liu L, Dharmadhikari S, et al. Partial decellularization eliminates immunogenicity in tracheal allografts. Bioeng Transl Med. 2023. https://doi.org/10.1002/btm2.10525.
Elliott MJ, De Coppi P, Speggiorin S, et al. Stem-cell-based, tissue engineered tracheal replacement in a child: a 2-year follow-up study. The Lancet. 2012;380:994-1000.
Chesterman P, Smith AU. Homotransplantation of articular cartilage and isolated chondrocytes: an experimental study in rabbits. J Bone Joint Surg Br. 1968;50:184-197.
Nair LS, Laurencin CT. Biodegradable polymers as biomaterials. Prog Polym Sci. 2007;32:762-798.
Dharmadhikari S, Liu L, Shontz K, et al. Deconstructing tissue engineered trachea: assessing the role of synthetic scaffolds, segmental replacement and cell seeding on graft performance. Acta Biomater. 2020;102:181-191.
Danielson A, Liu L, Shontz KM, et al. Spatial and temporal analysis of host cells in tracheal graft implantation. Laryngoscope. 2020;131:E340-E345. https://doi.org/10.1002/lary.28781.
Liu L, Stephens B, Bergman M, May A, Chiang T. Role of collagen in airway mechanics. Bioengineering. 2021;8:13.
Chan C, Liu L, Dharmadhikari S, et al. A multimodal approach to quantify chondrocyte viability for airway tissue engineering. Laryngoscope. 2023;133(3):512-520.
Dharmadhikari S, Best C, King N, et al. Mouse model of tracheal replacement with electrospun nanofiber scaffolds. Ann Otol Rhinol Laryngol. 2019;128:391-400.
Greaney AM, Niklason LE. The history of engineered tracheal replacements: interpreting the past and guiding the future. Tissue Eng Part B Rev. 2021;27:341-352.
Fukunishi T, Ong CS, He YJ, et al. Fast-degrading tissue-engineered vascular grafts Lead to increased extracellular matrix cross-linking enzyme expression. Tissue Eng Part A. 2021;27(21-22):1368-1375.
Romeo A, Easley J, Regan D, et al. Rotator cuff repair using a bioresorbable nanofiber interposition scaffold: a biomechanical and histologic analysis in sheep. J Shoulder Elbow Surg. 2022;31(2):402-412.
Matsushita H, Hayashi H, Nurminsky K, et al. Novel reinforcement of corrugated nanofiber tissue-engineered vascular graft to prevent aneurysm formation for arteriovenous shunts in an ovine model. J Vasc Surg. 2022;3:182-191.
Fukunishi T, Best CA, Ong CS, et al. Role of bone marrow mononuclear cell seeding for nanofiber vascular grafts. Tissue Eng Part A. 2018;24(1-2):135-144.
Da Silva D, Kaduri M, Poley M, et al. Biocompatibility, biodegradation and excretion of polylactic acid (PLA) in medical implants and theranostic systems. Chem Eng J. 2018;340:9-14.
Mishra N, Goyal AK, Khatri K, et al. Biodegradable polymer based particulate carrier (s) for the delivery of proteins and peptides. Antiinflamm Antiallergy Agents Med Chem. 2008;7:240-251.
Bartnikowski M, Dargaville TR, Ivanovski S, Hutmacher DW. Degradation mechanisms of polycaprolactone in the context of chemistry, geometry and environment. Prog Polym Sci. 2019;96:1-20.
Li W-J, Cooper JA Jr, Mauck RL, et al. Fabrication and characterization of six electrospun poly (α-hydroxy ester)-based fibrous scaffolds for tissue engineering applications. Acta Biomater. 2006;2:377-385.
Tian L, Prabhakaran MP, Ding X, Ramakrishna S. Biocompatibility evaluation of emulsion electrospun nanofibers using osteoblasts for bone tissue engineering. J Biomater Sci Polym ed. 2013;24:1952-1968.
Shi H, Tsai KHY, Ma D, et al. Controlled dual release of dihydrotestosterone and flutamide from polycaprolactone electrospun scaffolds accelerate burn wound healing. FASEB J. 2022;36:e22310.
Liu M, Luo G, Wang Y, et al. Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo. Int J Nanomedicine. 2017;12:6827-6840.
Genden EM, Miles BA, Harkin TJ, et al. Single-stage long-segment tracheal transplantation. Am J Transplant. 2021;21(10):3421-3427.
Shibuya M. Vascular endothelial growth factor (VEGF) and its receptor (VEGFR) signaling in angiogenesis: a crucial target for anti-and pro-angiogenic therapies. Genes Cancer. 2011;2(12):1097-1105.
Genden EM, Boros P, Liu J, Bromberg JS, Mayer L. Orthotopic tracheal transplantation in the murine model1. Transplantation. 2002;73(9):1420-1425.
Queiroga TL, Cataneo DC, Martins RH, Reis TA, Cataneo AJ. Mitomycin C in the endoscopic treatment of laryngotracheal stenosis: systematic review and proportional meta-analysis. Int Arch Otorhinolaryngol. 2020;24(1):e112-e124.
Athanasiou KA, Niederauer GG, Agrawal CM. Sterilization, toxicity, biocompatibility and clinical applications of polylactic acid/polyglycolic acid copolymers. Biomaterials. 1996;17(2):93-102.
Danielson A, Liu L, Shontz KM, et al. Spatial and temporal analysis of host cells in tracheal graft implantation. Laryngoscope. 2021;131(2):E340-E345.
Genden EM, Iskander AJ, Bromberg JS, Mayer L. Orthotopic tracheal allografts undergo reepithelialization with recipient-derived epithelium. Arch Otolaryngol Head Neck Surg. 2003;129(1):118-123.
Fan K, Qiao XW, Nie J, et al. Orthotopic and heterotopic tracheal transplantation model in studying obliterative bronchiolitis. Transpl Immunol. 2013;28(4):170-175.