Methods to Study NLR in Human Blood Cells.
ATP
Autoinflammatory diseases
IL-1β secretion
Primary monocytes
Redox
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
15
8
2023
pubmed:
14
8
2023
entrez:
14
8
2023
Statut:
ppublish
Résumé
Autoinflammatory diseases are a group of inherited and multifactorial disorders characterized by an over-activation of innate immune response. In most cases, the clinical manifestations are due to increased activity of the NLRP3 inflammasome resulting in increased IL-1β secretion. Investigating inflammatory cells from subjects affected by autoinflammatory diseases presents a number of technical difficulties related to the rarity of the diseases, to the young age of most patients, to the difficult modulation of gene expression in primary cells. However, since cell stress is involved in the pathophysiology of these diseases, the study of freshly drawn blood monocytes from patients affected by IL-1-mediated diseases strongly increases the chances that the observed phenomena is indeed pertinent to the pathogenesis of the disease and not influenced by the long-term cell culture conditions (e.g., the high O
Identifiants
pubmed: 37578719
doi: 10.1007/978-1-0716-3350-2_8
doi:
Substances chimiques
NLR Family, Pyrin Domain-Containing 3 Protein
0
Interleukin-1beta
0
Inflammasomes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115-122Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Harapas CR, Steiner A, Davidson S, Masters SL (2018) An update on autoinflammatory diseases: Inflammasomopathies. Rheumatol Rep 20:40
doi: 10.1007/s11926-018-0750-4
Mantovani A, Dinarello CA, Molgora M, Garlanda C (2019) Interleukin-1 and related cytokines in the regulation of inflammation and immunity. Immunity 50:778–795
doi: 10.1016/j.immuni.2019.03.012
pubmed: 30995499
pmcid: 7174020
Gattorno M, Martini A (2013) Beyond the NLRP3 inflammasome: autoinflammatory diseases reach adolescence. Arthritis Rheum 65:1137–1147
doi: 10.1002/art.37882
pubmed: 23400910
Carta S, Penco F, Lavieri R, Martini A, Dinarello CA, Gattorno M et al (2015) Cell stress increases ATP release in NLRP3 inflammasome-mediated autoinflammatory diseases, resulting in cytokine imbalance. Proc Natl Acad Sci U S A 112:2835–2840
doi: 10.1073/pnas.1424741112
pubmed: 25730877
pmcid: 4352822
Tassi S, Carta S, Delfino L, Caorsi R, Martini A, Gattorno M et al (2010) Altered redox state of monocytes from cryopyrin-associated periodic syndromes causes accelerated IL-1beta secretion. Proc Natl Acad Sci U S A 107:9789–9794
doi: 10.1073/pnas.1000779107
pubmed: 20445104
pmcid: 2906851
Carta S, Tassi S, Delfino L, Omenetti A, Raffa S, Torrisi MR et al (2012) Deficient production of IL-1 receptor antagonist and IL-6 coupled to oxidative stress in cryopyrin-associated periodic syndrome monocytes. Ann Rheum Dis 71:1577–1581
doi: 10.1136/annrheumdis-2012-201340
pubmed: 22753384
Andrei C, Dazzi C, Lotti L, Torrisi MR, Chimini G, Rubartelli A (1999) The secretory route of the leaderless protein interleukin 1beta involves exocytosis of endolysosome-related vesicles. Mol Biol Cell 10:1463–1475
doi: 10.1091/mbc.10.5.1463
pubmed: 10233156
pmcid: 25302
Andrei C, Margiocco P, Poggi A, Lotti LV, Torrisi MR, Rubartelli A (2004) Phospholipases C and A2 control lysosome-mediated IL-1 beta secretion: implications for inflammatory processes. Proc Natl Acad Sci U S A 101:9745–9750
doi: 10.1073/pnas.0308558101
pubmed: 15192144
pmcid: 470745
Carta S, Tassi S, Semino C, Fossati G, Mascagni P, Dinarello CA et al (2006) Histone deacetylase inhibitors prevent exocytosis of interleukin-1beta-containing secretory lysosomes: role of microtubules. Blood 108:1618–1626
doi: 10.1182/blood-2006-03-014126
pubmed: 16684958
pmcid: 1895509
Gattorno M, Tassi S, Carta S, Delfino L, Ferlito F, Pelagatti MA et al (2007) Pattern of interleukin-1beta secretion in response to lipopolysaccharide and ATP before and after interleukin-1 blockade in patients with CIAS1 mutations. Arthritis Rheum 56:3138–3148
doi: 10.1002/art.22842
pubmed: 17763411
Gattorno M, Piccini A, Lasiglie D, Tassi S, Brisca G, Carta S et al (2008) The pattern of response to anti-interleukin-1 treatment distinguishes two subsets of patients with systemic-onset juvenile idiopathic arthritis. Arthritis Rheum 58:1505–1615
doi: 10.1002/art.23437
pubmed: 18438814
Carta S, Tassi S, Pettinati I, Delfino L, Dinarello CA, Rubartelli A (2011) The rate of interleukin-1beta secretion in different myeloid cells varies with the extent of redox response to toll-like receptor triggering. J Biol Chem 286:27069–27080
doi: 10.1074/jbc.M110.203398
pubmed: 21628463
pmcid: 3149300
Omenetti A, Carta S, Delfino L, Martini A, Gattorno M, Rubartelli A (2014) Increased NLRP3-dependent interleukin 1beta secretion in patients with familial Mediterranean fever: correlation with MEFV genotype. Ann Rheum Dis 73:462–469
doi: 10.1136/annrheumdis-2012-202774
pubmed: 23505242
Lavieri R, Piccioli P, Carta S, Delfino L, Castellani P, Rubartelli A (2014) TLR costimulation causes oxidative stress with unbalance of proinflammatory and anti-inflammatory cytokine production. J Immunol 192:5373–5381
doi: 10.4049/jimmunol.1303480
pubmed: 24771848
Tassi S, Carta S, Vene R, Delfino L, Ciriolo MR, Rubartelli A (2009) Pathogen-induced interleukin-1beta processing and secretion is regulated by a biphasic redox response. J Immunol 183:1456–1462
doi: 10.4049/jimmunol.0900578
pubmed: 19561107