Asymmetric charge balanced waveforms direct retinal ganglion cell axon growth.

Humans Retinal Ganglion Cells / physiology Axons / physiology Nerve Regeneration / physiology Glaucoma / therapy Blindness Optic Nerve Injuries / therapy

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
14 08 2023
Historique:
received: 13 02 2023
accepted: 04 08 2023
medline: 16 8 2023
pubmed: 15 8 2023
entrez: 14 8 2023
Statut: epublish

Résumé

Failure to direct axon regeneration to appropriate targets is a major barrier to restoring function after nerve injury. Development of strategies that can direct targeted regeneration of neurons such as retinal ganglion cells (RGCs) are needed to delay or reverse blindness in diseases like glaucoma. Here, we demonstrate that a new class of asymmetric, charge balanced (ACB) waveforms are effective at directing RGC axon growth, in vitro, without compromising cell viability. Unlike previously proposed direct current (DC) stimulation approaches, charge neutrality of ACB waveforms ensures the safety of stimulation while asymmetry ensures its efficacy. Furthermore, we demonstrate the relative influence of pulse amplitude and pulse width on the overall effectiveness of stimulation. This work can serve as a practical guideline for the potential deployment of electrical stimulation as a treatment strategy for nerve injury.

Identifiants

DOI: 10.1038/s41598-023-40097-6 PMID: 37580344 PMC: PMC10425404
pubmed: 37580344
doi: 10.1038/s41598-023-40097-6
pii: 10.1038/s41598-023-40097-6
pmc: PMC10425404
doi:

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

13233

Subventions

Organisme : NEI NIH HHS
ID : K08 EY031797
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY029220
Pays : United States

Informations de copyright

© 2023. Springer Nature Limited.

Références

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Auteurs

M G Peng (MG)

Department of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, USA.

E Iseri (E)

Department of Electrical and Computer Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.

A Simonyan (A)

Department of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, USA.

P Lam (P)

Department of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, USA.

T Kim (T)

Department of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, USA.

S Medvidovic (S)

Department of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, USA.

J Paknahad (J)

Department of Electrical and Computer Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.

M Machnoor (M)

Department of Electrical and Computer Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.

G Lazzi (G)

Department of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, USA.
Department of Electrical and Computer Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.

K K Gokoffski (KK)

Department of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, USA. kimberly.gokoffski@med.us.edu.
ORCID: 0000-0003-3589-0296

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Asymmetric charge balanced waveforms direct...
questionsmedicales.fr › Cellules › Neurones › Neurones afférents › Neurones rétiniens › Cellules ganglionnaires rétiniennes › Asymmetric charge balanced waveforms direct...
questionsmedicales.fr › Cellules › Neurones › Neurofibres › Axones › Asymmetric charge balanced waveforms direct...
questionsmedicales.fr › Phénomènes biologiques › Régénération › Régénération nerveuse › Asymmetric charge balanced waveforms direct...
questionsmedicales.fr › Maladies de l'oeil › Hypertension oculaire › Glaucome › Asymmetric charge balanced waveforms direct...
questionsmedicales.fr › États, signes et symptômes pathologiques › Signes et symptômes › Manifestations neurologiques › Troubles sensitifs › Troubles de la vision › Cécité › Asymmetric charge balanced waveforms direct...
questionsmedicales.fr › Plaies et blessures › Traumatismes du système nerveux › Traumatismes cranioencéphaliques › Lésions traumatiques des nerfs crâniens › Lésions traumatiques du nerf optique › Asymmetric charge balanced waveforms direct...