Rhizopus stolonifer biomass catalytic transesterification capability: optimization of cultivation conditions.

Biomass Esterification Fatty Acids Rhizopus / metabolism Lipase / metabolism Biofuels / microbiology
Biocatalysis Biodiesel Fungal biomass Rhizopus Transesterification Triglycerides Whole-cell

Journal

Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812

Informations de publication

Date de publication:
14 Aug 2023
Historique:
received: 28 04 2023
accepted: 01 07 2023
medline: 16 8 2023
pubmed: 15 8 2023
entrez: 14 8 2023
Statut: epublish

Résumé

Using fungal biomass for biocatalysis is a potential solution for the expensive cost of the use o enzymes. Production of fungal biomass with effective activity requires optimizing the cultivation conditions. Rhizopus stolonifer biomass was optimized for transesterification and hydrolysis of waste frying oil (WFO). Growth and biomass lipolytic activities of R. stolonifer improved under shaking conditions compared to static conditions, and 200 rpm was optimum. As biomass lipase and transesterification activities inducer, olive oil was superior to soybean, rapeseed, and waste frying oils. Biomass produced in culture media containing fishmeal as an N-source feedstock had higher lipolytic capabilities than corn-steep liquor and urea. Plackett Burman screening of 9 factors showed that pH (5-9), fishmeal (0.25-1.7%, w/v), and KH Optimized culture conditions improved the whole cell transesterification capability of Rhizopus stolonifer biomass in terms of fatty acid methyl ester (FAME) concentration by 67.65% to a final FAME concentration of 85.5%, w/w.

Sections du résumé

BACKGROUND BACKGROUND
Using fungal biomass for biocatalysis is a potential solution for the expensive cost of the use o enzymes. Production of fungal biomass with effective activity requires optimizing the cultivation conditions.
RESULTS RESULTS
Rhizopus stolonifer biomass was optimized for transesterification and hydrolysis of waste frying oil (WFO). Growth and biomass lipolytic activities of R. stolonifer improved under shaking conditions compared to static conditions, and 200 rpm was optimum. As biomass lipase and transesterification activities inducer, olive oil was superior to soybean, rapeseed, and waste frying oils. Biomass produced in culture media containing fishmeal as an N-source feedstock had higher lipolytic capabilities than corn-steep liquor and urea. Plackett Burman screening of 9 factors showed that pH (5-9), fishmeal (0.25-1.7%, w/v), and KH
CONCLUSION CONCLUSIONS
Optimized culture conditions improved the whole cell transesterification capability of Rhizopus stolonifer biomass in terms of fatty acid methyl ester (FAME) concentration by 67.65% to a final FAME concentration of 85.5%, w/w.

Identifiants

DOI: 10.1186/s12934-023-02141-y PMID: 37580714 PMC: PMC10424374
pubmed: 37580714
doi: 10.1186/s12934-023-02141-y
pii: 10.1186/s12934-023-02141-y
pmc: PMC10424374
doi:

Substances chimiques

Fatty Acids 0
Lipase EC 3.1.1.3
Biofuels 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

154

Informations de copyright

© 2023. BioMed Central Ltd., part of Springer Nature.

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Auteurs

Nadeem I Elhussieny (NI)

Department of Life Science and Chemistry, Constructor University, Campus Ring 1, 28759, Bremen, Germany. nelhussieny@constructor.university.
Department of Chemistry of Natural and Microbial Products, National Research Centre, Cairo, 12622, Egypt. nelhussieny@constructor.university.
Institute of Environmental Biology and Biotechnology, University of Applied Sciences, 28199, Bremen, Germany. nelhussieny@constructor.university.

Heba A El-Refai (HA)

Department of Chemistry of Natural and Microbial Products, National Research Centre, Cairo, 12622, Egypt.

Sayeda S Mohamed (SS)

Department of Chemistry of Natural and Microbial Products, National Research Centre, Cairo, 12622, Egypt.

Yousseria M Shetaia (YM)

Department of Microbiology, Ain Shams University, Abbassia, Cairo, 11566, Egypt.

Hala A Amin (HA)

Department of Chemistry of Natural and Microbial Products, National Research Centre, Cairo, 12622, Egypt.

Gerd Klöck (G)

Institute of Environmental Biology and Biotechnology, University of Applied Sciences, 28199, Bremen, Germany.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biomasse › Rhizopus stolonifer biomass catalytic...
questionsmedicales.fr › Métabolisme › Estérification › Rhizopus stolonifer biomass catalytic...
questionsmedicales.fr › Lipides › Acides gras › Rhizopus stolonifer biomass catalytic...
questionsmedicales.fr › Eucaryotes › Champignons › Champignons non classés › Mucorales › Rhizopus › Rhizopus stolonifer biomass catalytic...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Carboxylic ester hydrolases › Triacylglycerol lipase › Rhizopus stolonifer biomass catalytic...
questionsmedicales.fr › Environnement et santé publique › Environnement › Ressources de production d'énergie › Énergie renouvelable › Biocarburants › Rhizopus stolonifer biomass catalytic...