Screening of immunogenic proteins and evaluation of vaccine candidates against Mycoplasma synoviae.
Journal
NPJ vaccines
ISSN: 2059-0105
Titre abrégé: NPJ Vaccines
Pays: England
ID NLM: 101699863
Informations de publication
Date de publication:
15 Aug 2023
15 Aug 2023
Historique:
received:
04
03
2023
accepted:
03
08
2023
medline:
16
8
2023
pubmed:
16
8
2023
entrez:
15
8
2023
Statut:
epublish
Résumé
Mycoplasma synoviae (M. synoviae) is a serious avian pathogen that causes significant economic losses to chicken and turkey producers worldwide. The currently available live attenuated and inactivated vaccines provide limited protection. The objective of this study was to identify potential subunit vaccine candidates using immunoproteomics and reverse vaccinology analyses and to evaluate their preliminary protection. Twenty-four candidate antigens were identified, and five of them, namely RS01790 (a putative sugar ABC transporter lipoprotein), BMP (a substrate-binding protein of the BMP family ABC transporter), GrpE (a nucleotide exchange factor), RS00900 (a putative nuclease), and RS00275 (an uncharacterized protein), were selected to evaluate their immunogenicity and preliminary protection. The results showed that all five antigens had good immunogenicity, and they were localized on the M. synoviae cell membrane. The antigens induced specific humoral and cellular immune responses, and the vaccinated chickens exhibited significantly greater body weight gain and lower air sac lesion scores and tracheal mucosal thicknesses. Additionally, the vaccinated chickens had lower M. synoviae loads in throat swabs than non-vaccinated chickens. The protective effect of the RS01790, BMP, GrpE, and RS00900 vaccines was better than that of the RS00275 vaccine. In conclusion, our study demonstrates the potential of subunit vaccines as a new approach to developing M. synoviae vaccines, providing new ideas for controlling the spread of M. synoviae worldwide.
Identifiants
pubmed: 37582795
doi: 10.1038/s41541-023-00721-y
pii: 10.1038/s41541-023-00721-y
pmc: PMC10427712
doi:
Types de publication
Journal Article
Langues
eng
Pagination
121Subventions
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 31672516
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 32172802
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 31172300
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 30670079
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 32002301
Organisme : China Postdoctoral Science Foundation
ID : 2019M661953
Informations de copyright
© 2023. Springer Nature Limited.
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