Pathogenicity characterization of nocardiosis in orange-spotted grouper (Epinephelus coioides) via exposure to nine distinct isolates of Nocardia seriolae and four administrative routes.
administrative routes
chronic disease
granulomatous
nocardiosis
orange-spotted grouper
pathogenicity
Journal
Journal of fish diseases
ISSN: 1365-2761
Titre abrégé: J Fish Dis
Pays: England
ID NLM: 9881188
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
revised:
20
07
2023
received:
05
05
2023
accepted:
22
07
2023
pubmed:
16
8
2023
medline:
16
8
2023
entrez:
16
8
2023
Statut:
ppublish
Résumé
Nocardia seriolae causes chronic nocardiosis in various marine and freshwater aquatic animals; however, grouper species have rarely been investigated. This study evaluated the pathogenicity of nocardiosis following N. seriolae infection in the orange-spotted grouper Epinephelus coioides. Nine identified genetic isolates of N. seriolae were tested in vivo using the intraperitoneal method and observed daily for 35 days. The most virulent isolate was then used to evaluate transmission through different routes (intraperitoneal IP, intramuscular IM, oral OR, and immersion IS) in the same fish model and was observed daily for 42 days. The results showed mild variation in virulence among N. seriolae isolates. AOD107132-2 K and OT103003-N11 strains displayed the highest and lowest risk virulence, respectively, based on the accumulation and kinetics of mortality. IM and IP administrations showed an early phase response with early mortality by 5 dpc (30%-100%), while slower kinetics of nocardiosis occurred in the OR and IS routes with slow mortality at 35 dpc (4%-8%). Histopathology revealed typical granulomas, confirming the progression of nocardiosis in the diseased fish. These results provide the basis for further studies on the virulence profile of N. seriolae in Taiwan and a well-suited route of administration in orange-spotted groupers for further prevention development.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1257-1268Subventions
Organisme : Council of Agriculture, Taiwan
Informations de copyright
© 2023 John Wiley & Sons Ltd.
Références
American Society for Microbiology. (1957). Staining methods. In Manual of microbiological methods (pp. 10-36). McGraw-Hill.
Bafghi, M. F., Eshraghi, S. S., Heidarieh, P., Habibnia, S., & Nasab, M. R. (2014). DNA extraction from Nocardia species for special genes analysis using PCR. North American Journal of Medical Sciences, 6, 231-233.
Beaman, B. L., & Beaman, L. (1994). Nocardia species: Host-parasite relationships. Clinical Microbiology Reviews, 7, 213-264.
Chen, S. C., Lee, J. L., Lai, C. C., Gu, Y. W., Wang, C. T., Chang, H. Y., & Tsai, K. H. (2000). Nocardiosis in sea bass, Lateolabrax japonicus, in Taiwan. Journal of Fish Diseases, 23, 299-307.
Delamare-Deboutteville, J., Bowater, R., Condon, K., Reynolds, A., Fisk, A., Aviles, F., & Barnes, A. C. (2015). Infection and pathology in Queensland grouper, Epinephelus lanceolatus, (Bloch), caused by exposure to Streptococcus agalactiae via different routes. Journal of Fish Diseases, 38, 1021-1035.
Elkesh, A., Kantham, K. P. L., Shinn, A. P., Crumlish, M., & Richards, R. H. (2013). Systemic nocardiosis in a Mediterranean population of cultured meagre, Argyrosomus regius Asso (Perciformes: Sciaenidae). Journal of Fish Diseases, 36, 141-149.
Huang, X., Liu, S., Chen, X., Zhang, H., Yao, J., Geng, Y., Ou, Y., Chen, D., Yin, L., Li, L., & Yang, S. (2021). Comparative pathological description of nocardiosis in largemouth bass (Micropterus salmoides) and other Perciformes. Aquaculture, 534, 736193.
Itano, T., Kawakami, H., Kono, T., & Sakai, M. (2006). Experimental induction of nocardiosis in yellowtail, Seriola quinqueradiata Temminck & Schlegel by artificial challenge. Journal of Fish Diseases, 29, 529-534.
Itano, T., Nakaoka, N., Kawakami, H., Kono, T., & Sakai, M. (2006). Comparison of sensitivity between yellowtail Seriola quinqueradiata and red sea bream Pagrus major to Nocardia seriolae. Fish Pathology, 41, 135-139.
Kato, G., Oka, K., Matsumoto, M., Kanemaru, M., Yamamoto, M., & Sano, M. (2020). Prevalence of infection with Nocardia seriolae in juvenile of yellowtail Seriola quinqueradiata cultured in Owase Bay, Japan. Fish Pathology, 55, 1-7.
Kudo, T., Hatai, K., & Seino, A. (1988). Nocardia seriolae sp. nov. causing nocardiosis of cultured fish. International Journal of Systematic Bacteriology, 38, 173-178.
Lee, N.-S., Han, H.-J., Kim, M.-S., Do, J.-W., Jung, S.-H., Cho, H.-I., & Kim, J.-D. (2016). Artificial infection with Nocardia seriolae and the histological examination at snakehead Channa argus. Journal of Fisheries and Marine Sciences Education, 28, 653-660.
Liao, P., Tsai, M., See, M. S., Wang, P., & Chen, S. (2021). Isolation and characterization of Nocardia seriolae, a causative agent of systematic granuloma in cultured east Asian four finger threadfin, Eleutheronema rhadinum, and red snapper, Lutjanus erythropterus. Aquaculture Research, 52, 763-770.
Liao, P. C., Tsai, M. A., See, M. S., Wang, P. C., & Chen, S. C. (2020). Isolation and characterization of Nocardia seriolae, a causative agent of systematic granuloma in cultured East Asian four-finger threadfin, Eleutheronema rhadinum, and red snapper, Lutjanus erythropterus. Aquaculture Research, 52, 763-770.
Miyoshi, Y., & Suzuki, S. (2003). A PCR method to detect Nocardia seriolae in fish samples. Fish Pathology, 38, 93-97.
Murphy, F. A., Gibbs, E. P. J., Horzinek, M. C., & Studdert, M. J. (1999). Determinants of viral virulence and host resistance/ susceptibility. In Veterinary virology (p. 112). Academic Press.
Nebendahl, K. (2012). Chapter. Routes of administration. In The laboratory mouse (pp. 709-725). Academic Press.
Nguyen, P., Nazareth, S. C., Chen, M. Y., Wang, P. C., & Chen, S. C. (2023). An epidemiological analysis of Nocardia seriolae isolated from a wide range of aquatic animals in Taiwan, based on their genotype and enzymatic activity. Journal of Fish Diseases, 46, 1-14.
Nguyen, P., Nazareth, S. C., Chen, M. Y., Wang, P. C., & Chen, S. C. (2023). An epidemiological analysis of Nocardia seriolae isolated from a wide range of aquatic animals in Taiwan, based on their genotype and enzymatic activity. Journal of Fish Diseases, 46, 1-14.
OECD. (2016). Bacteria: Pathogenicity factors. In Safety assessment of transgenic organisms: OECD consensus documents (pp. 27-79). OECD Publishing.
Peterson, J. W. (1996). Bacterial pathogenesis. In S. Baron (Ed.), Medical Microbiology (pp. 3-4). University of Texas Medical Branch at Galveston.
Shimahara, Y., Huang, Y. F., Tsai, M. A., Wang, P. C., Yoshida, T., Lee, J. L., & Chen, S. C. (2009). Genotypic and phenotypic analysis of fish pathogen, Nocardia seriolae, isolated in Taiwan. Aquaculture, 294, 165-171.
Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (2011). Administration of substances to laboratory animals: Routes of administration and factors to consider. Journal of the American Association for Laboratory Animal Science, 50, 600-613.
van Seventer, J. M., & Hochberg, N. S. (2017). Principles of infectious diseases: Transmission, diagnosis, prevention, and control (pp. 22-39). International Encyclopedia of Public Health. Elsevier.
Wang, F., Wang, X. G., Liu, C., Chang, O. Q., Feng, Y. Y., Jiang, L., & Li, K. B. (2017b). Transparent Tiger barb Puntius tetrazona, a fish model for in vivo analysis of nocardial infection. Veterinary Microbiology, 211, 67-73.
Wang, F., Wang, X., Liu, C., Chang, O., Feng, Y., Jiang, L., & Li, K. (2017a). Nocardia seriolae infection in cultured jade perch, Scortum barcoo. Aquaculture International, 25, 2201-2212.
Wang, G. L., Xu, Y. J., Jin, S., Zhu, J. L., & Yuan, S. P. (2007). Nocardiosis in snakehead, Ophiocephalus argus cantor. Aquaculture, 271, 54-60.
Wang, G. L., Yuan, S. P., & Jin, S. (2005). Nocardiosis in large yellow croaker, Larimichthys crocea (Richardson). Journal of Fish Diseases, 28, 339-345.
Wang, P. C., Chen, S. D., Tsai, M. A., Weng, Y. J., Chu, S. Y., Chern, R. S., & Chen, S. C. (2009). Nocardia seriolae infection in the three striped tigerfish, Terapon jarbua (Forsskål). Journal of Fish Diseases, 32, 301-310.
Wang, P.-C., Tsai, M.-A., Liang, Y.-C., Chan, Y., & Chen, S. C. (2014). Nocardia seriolae, a causative agent of systemic granuloma in spotted butterfish, Scatophagus argus, Linn. African Journal of Microbiology Research, 6, 6348-6353.
Xueping, L., Ruoxuan, Z., & Yi, G. (2020). Nocardia seriolae: A serious threat to the largemouth bass Micropterus salmoides industry in Southwest China. Diseases of Aquatic Organisms, 142, 13-21.