Side-by-side comparison of the two widely studied GRPR radiotracers, radiolabeled NeoB and RM2, in a preclinical setting.


Journal

European journal of nuclear medicine and molecular imaging
ISSN: 1619-7089
Titre abrégé: Eur J Nucl Med Mol Imaging
Pays: Germany
ID NLM: 101140988

Informations de publication

Date de publication:
Nov 2023
Historique:
received: 28 04 2023
accepted: 24 07 2023
medline: 30 10 2023
pubmed: 16 8 2023
entrez: 16 8 2023
Statut: ppublish

Résumé

NeoB and RM2 are the most investigated gastrin-releasing peptide receptor (GRPR)-targeting radiotracers in preclinical and clinical studies. Therefore, an extensive side-by-side comparison of the two radiotracers is valuable to demonstrate whether one has advantages over the other. Accordingly, this study aims to compare the in vitro and in vivo characteristics of radiolabeled NeoB and RM2 to guide future clinical studies. The stability of the radiolabeled GRPR analogs was determined in phosphate buffered saline (PBS), and commercially available mouse and human serum. Target affinity was determined by incubating human prostate cancer PC-3 cells with [ Both radiotracers demonstrated high stability in PBS and human serum, but stability in mouse serum decreased substantially over time. Moreover, both radiotracers demonstrated high GRPR affinity and specificity, but a higher uptake of [ Based on these findings, we conclude that the in vivo tumor-targeting capability of radiolabeled NeoB and RM2 is similar. Additional studies are needed to determine whether the differences observed in physiological organ uptakes, i.e., the pancreas, kidneys, and blood, result in relevant differences in organ absorbed doses when the radiotracers are applied for therapeutic purposes.

Identifiants

pubmed: 37584725
doi: 10.1007/s00259-023-06364-4
pii: 10.1007/s00259-023-06364-4
pmc: PMC10611828
doi:

Substances chimiques

Bombesin PX9AZU7QPK
Receptors, Bombesin 0

Types de publication

Comparative Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

3851-3861

Subventions

Organisme : NWO ZonMw Veni
ID : 09150161810061

Informations de copyright

© 2023. The Author(s).

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Auteurs

T S T Damiana (TST)

Department of Radiology & Nuclear Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.

P Paraïso (P)

Department of Radiology & Nuclear Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.

C de Ridder (C)

Department of Experimental Urology, Erasmus Medical Center, Rotterdam, The Netherlands.

D Stuurman (D)

Department of Radiology & Nuclear Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.

Y Seimbille (Y)

Department of Radiology & Nuclear Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.

S U Dalm (SU)

Department of Radiology & Nuclear Medicine, Erasmus Medical Center, Rotterdam, The Netherlands. s.dalm@erasmusmc.nl.

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Classifications MeSH