Axonal transport of Frizzled5 by Alcadein α-containing vesicles is associated with kinesin-1.
Journal
Molecular biology of the cell
ISSN: 1939-4586
Titre abrégé: Mol Biol Cell
Pays: United States
ID NLM: 9201390
Informations de publication
Date de publication:
01 10 2023
01 10 2023
Historique:
medline:
22
9
2023
pubmed:
16
8
2023
entrez:
16
8
2023
Statut:
ppublish
Résumé
Alcadein α (Alcα) and amyloid-β protein precursor (APP) are cargo receptors that associate vesicles with kinesin-1. These vesicles, which contain either Alcα or APP, transport various proteins/cargo molecules into axon nerve terminals. Here, we analyzed immune-isolated Alcα- and APP-containing vesicles of adult mouse brains with LC-MS/MS and identified proteins present in vesicles that contained either Alcα or APP. Among these proteins, Frizzled-5 (Fzd5), a Wnt receptor, was detected mainly in Alcα vesicles. Although colocalization ratios of Fzd5 with Alcα are low in the neurites of differentiating neurons by a low expression of Fzd5 in embryonic brains, the suppression of Alcα expression decreased the localization of Fzd5 in neurites of primary cultured neurons. Furthermore, Fzd5-EGFP expressed in primary cultured neurons was preferentially transported in axons with the transport velocities of Alcα vesicles. In synaptosomal fractions of adult-mice brains that express higher levels of Fzd5, the amount of Fzd5 and the phosphorylation level of calcium/calmodulin-dependent protein kinase-II were reduced in the Alcα-deficient mice. These results suggest that reduced transport of Fzd5 by Alcα-containing vesicles associated with kinesin-1 in axon terminals may impair the response to Wnt ligands in the noncanonical Ca
Identifiants
pubmed: 37585286
doi: 10.1091/mbc.E22-10-0495
pmc: PMC10559311
doi:
Substances chimiques
Amyloid beta-Protein Precursor
0
Kinesins
EC 3.6.4.4
Clstn1 protein, mouse
0
Fzd5 protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
ar110Références
J Biol Chem. 2003 Dec 5;278(49):49448-58
pubmed: 12972431
Protein Sci. 2022 Jan;31(1):8-22
pubmed: 34717010
FEBS Lett. 2018 Aug;592(16):2716-2724
pubmed: 30055048
Ann Neurol. 2011 Jun;69(6):1026-31
pubmed: 21681798
Nat Rev Neurosci. 2017 May;18(5):281-298
pubmed: 28360418
J Alzheimers Dis. 2014;39(4):861-70
pubmed: 24305499
Development. 2010 Jul;137(13):2215-25
pubmed: 20530549
Cell Tissue Res. 2015 Jan;359(1):215-23
pubmed: 25234280
Biochem Biophys Res Commun. 2000 Jun 16;272(3):895-9
pubmed: 10860848
Hum Mol Genet. 2012 Jul 1;21(13):2845-54
pubmed: 22434822
Cereb Cortex. 2020 Jun 1;30(7):4044-4063
pubmed: 32219307
Dev Cell. 2015 Feb 23;32(4):502-15
pubmed: 25710536
Mol Biol Cell. 2017 Dec 15;28(26):3844-3856
pubmed: 29093024
J Cell Biol. 2001 Mar 5;152(5):959-70
pubmed: 11238452
PLoS One. 2013 Oct 18;8(10):e78892
pubmed: 24205342
PLoS One. 2012;7(8):e43058
pubmed: 22905201
J Cell Biol. 1980 Sep;86(3):831-45
pubmed: 7410481
Proteomics. 2010 Nov;10(21):3775-88
pubmed: 20925061
Nat Neurosci. 2012 Jan 29;15(3):349-57
pubmed: 22286176
Exp Brain Res. 2012 Apr;217(3-4):435-40
pubmed: 22006270
J Biol Chem. 2009 Dec 25;284(52):36024-36033
pubmed: 19864413
J Neurosci. 2021 Jun 16;41(24):5157-5172
pubmed: 33926999
Mol Cell Neurosci. 2002 Nov;21(3):393-409
pubmed: 12498782
J Cell Biol. 1989 Apr;108(4):1453-63
pubmed: 2522455
Mol Biol Cell. 2017 Dec 15;28(26):3857-3869
pubmed: 29093025
Nat Rev Mol Cell Biol. 2009 Nov;10(11):765-77
pubmed: 19851335
Proc Natl Acad Sci U S A. 2016 Mar 1;113(9):2418-23
pubmed: 26884162
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Proc Natl Acad Sci U S A. 1990 Feb;87(3):1061-5
pubmed: 1689058
J Neurosci. 2005 Feb 2;25(5):1219-25
pubmed: 15689559
Development. 2016 Mar 15;143(6):994-1004
pubmed: 26839361
Mol Biol Cell. 2006 Aug;17(8):3651-63
pubmed: 16760430
Traffic. 2012 Jun;13(6):834-48
pubmed: 22404616
Brain Res. 2016 Sep 1;1646:227-234
pubmed: 27268412
PLoS One. 2012;7(3):e33943
pubmed: 22470497
EMBO J. 2021 Jun 15;40(12):e107471
pubmed: 34008862
Biochim Biophys Acta Mol Cell Res. 2018 Jul 17;1865(10):1397-1409
pubmed: 30021127
Neuroscience. 2015 Feb 12;286:87-96
pubmed: 25463516
Mol Neurodegener. 2010 Sep 15;5:35
pubmed: 20843325
J Neurosci. 2009 Nov 18;29(46):14534-44
pubmed: 19923287
Traffic. 2009 May;10(5):572-89
pubmed: 19192245
J Biol Chem. 2004 Jun 4;279(23):24343-54
pubmed: 15037614
PLoS One. 2013 Apr 26;8(4):e62431
pubmed: 23658629
J Proteome Res. 2008 Feb;7(2):731-40
pubmed: 18183947
EMBO Mol Med. 2023 May 8;15(5):e17052
pubmed: 36994913
Mol Med. 1997 Feb;3(2):111-23
pubmed: 9085254
Mol Biol Cell. 2014 Nov 5;25(22):3569-80
pubmed: 25165140
Neuron. 2000 Nov;28(2):449-59
pubmed: 11144355
Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):6158-62
pubmed: 8650236
J Biol Chem. 2008 Oct 31;283(44):29633-7
pubmed: 18650433
EMBO J. 2007 Mar 21;26(6):1475-86
pubmed: 17332754
Traffic. 2014 Jan;15(1):1-11
pubmed: 24102769
Cold Spring Harb Perspect Med. 2012 Jul;2(7):a006338
pubmed: 22762015
Cell. 1985 Aug;42(1):39-50
pubmed: 3926325
EMBO J. 2018 Jun 1;37(11):
pubmed: 29661886
Anal Biochem. 1986 Mar;153(2):262-6
pubmed: 3706709
EMBO J. 2011 Nov 16;30(22):4523-38
pubmed: 21915095
J Biol Chem. 2008 Jul 4;283(27):19119-31
pubmed: 18468999
Alzheimers Dement (N Y). 2019 Nov 07;5:740-750
pubmed: 31754625
Trends Mol Med. 2023 Jul;29(7):487-488
pubmed: 37169660
J Biol Chem. 2020 Jul 10;295(28):9650-9662
pubmed: 32467230