Immunomodulatory effects of one novel microRNA miR-63 in pearl oyster Pinctada fucata martensii.

Animals MicroRNAs / metabolism Pinctada Gene Expression Profiling / veterinary Antioxidants / metabolism Immunity
Pinctada fucata martensii Pm-novel-miR-63 Transplant immunization

Journal

Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220

Informations de publication

Date de publication:
Sep 2023
Historique:
received: 27 05 2023
revised: 13 08 2023
accepted: 14 08 2023
medline: 28 8 2023
pubmed: 17 8 2023
entrez: 16 8 2023
Statut: ppublish

Résumé

Novel microRNA miR-63 (novel-miR-63) from pearl oyster Pinctada fucata martensii (Pm-novel-miR-63) is a species-specific miRNA. Our previous research has shown that the expression of Pm-novel-miR-63 was significantly downregulated at 24 h after nucleus transplantation. In this study, we analyzed the function and regulatory role of Pm-novel-miR-63 in the immune response of pearl oysters. The results showed that Pm-novel-miR-63 expression increased after the stimulation of pathogen associated molecular patterns at 6-12 h, and the activity of immune and antioxidant enzymes in the serum decreased after Pm-novel-miR-63 overexpression. Transcriptome analysis revealed that Pm-novel-miR-63 participated in regulating transplantation immunity through the Notch and mRNA surveillance signaling pathways. Target prediction and dual luciferase analysis revealed that Pm-GDP-FucTP, Pm-CysLTR2, and Pm-RLR were the target genes of Pm-novel-miR-63. These results suggested that Pm-novel-miR-63 participated in regulating the immune response in pearl oysters and can serve as a new interference target to reasonably control excessive immune rejection in pearl culture.

Identifiants

DOI: 10.1016/j.fsi.2023.109002 PMID: 37586600
pubmed: 37586600
pii: S1050-4648(23)00488-6
doi: 10.1016/j.fsi.2023.109002
pii:
doi:

Substances chimiques

MicroRNAs 0
Antioxidants 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109002

Informations de copyright

Copyright © 2023. Published by Elsevier Ltd.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors have no competing interests to declare.

Auteurs

Meiqi Dai (M)

Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China.

Yuting Zhang (Y)

Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China.

Yu Jiao (Y)

Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang, 524088, China. Electronic address: jiaoy@gdou.edu.cn.

Yuewen Deng (Y)

Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang, 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, China; Guangdong Marine Ecology Early Warning and Monitoring Laboratory, Zhanjiang, 524088, China.

Xiaodong Du (X)

Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang, 524088, China.

Chuangye Yang (C)

Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Marine Ecology Early Warning and Monitoring Laboratory, Zhanjiang, 524088, China.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Immunomodulatory effects of one novel microRNA...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Immunomodulatory effects of one novel microRNA...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Mollusca › Bivalvia › Pinctada › Immunomodulatory effects of one novel microRNA...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de profil d'expression de gènes › Immunomodulatory effects of one novel microRNA...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents protecteurs › Antioxydants › Immunomodulatory effects of one novel microRNA...
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunomodulatory effects of one novel microRNA...