Reduced excitatory neurotransmission in the hippocampus after inflammation and sevoflurane anaesthesia.
excitatory neurotransmission
lipopolysaccharide
mEPSC
perioperative neurocognitive disorders
sevoflurane
synaptic plasticity
Journal
BJA open
ISSN: 2772-6096
Titre abrégé: BJA Open
Pays: England
ID NLM: 9918419157906676
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
24
02
2023
revised:
29
03
2023
accepted:
21
04
2023
medline:
17
8
2023
pubmed:
17
8
2023
entrez:
17
8
2023
Statut:
epublish
Résumé
Inflammation and general anaesthesia likely contribute to perioperative neurocognitive disorders, possibly by causing a neuronal imbalance of excitation and inhibition. We showed previously that treatment with lipopolysaccharide (LPS) and sevoflurane causes a sustained increase in a tonic inhibitory conductance in the hippocampus; however, whether excitatory neurotransmission is also altered remains unknown. The goal of this study was to examine excitatory synaptic currents in the hippocampus after treatment with LPS and sevoflurane. Synaptic plasticity in the hippocampus, a cellular correlate of learning and memory, was also studied. Mice were injected with vehicle or LPS (1 mg kg The amplitude of miniature excitatory postsynaptic currents (EPSCs) was reduced after LPS+sevoflurane ( The reduced amplitude of miniature EPSCs, coupled with the previously reported increase in tonic inhibition, indicates that the combination of LPS and sevoflurane markedly disrupts the balance of excitation and inhibition. Restoring this balance by pharmacologically enhancing excitatory neurotransmission and inhibiting the tonic current may represent an effective therapeutic option for perioperative neurocognitive disorders.
Sections du résumé
Background
UNASSIGNED
Inflammation and general anaesthesia likely contribute to perioperative neurocognitive disorders, possibly by causing a neuronal imbalance of excitation and inhibition. We showed previously that treatment with lipopolysaccharide (LPS) and sevoflurane causes a sustained increase in a tonic inhibitory conductance in the hippocampus; however, whether excitatory neurotransmission is also altered remains unknown. The goal of this study was to examine excitatory synaptic currents in the hippocampus after treatment with LPS and sevoflurane. Synaptic plasticity in the hippocampus, a cellular correlate of learning and memory, was also studied.
Methods
UNASSIGNED
Mice were injected with vehicle or LPS (1 mg kg
Results
UNASSIGNED
The amplitude of miniature excitatory postsynaptic currents (EPSCs) was reduced after LPS+sevoflurane (
Conclusions
UNASSIGNED
The reduced amplitude of miniature EPSCs, coupled with the previously reported increase in tonic inhibition, indicates that the combination of LPS and sevoflurane markedly disrupts the balance of excitation and inhibition. Restoring this balance by pharmacologically enhancing excitatory neurotransmission and inhibiting the tonic current may represent an effective therapeutic option for perioperative neurocognitive disorders.
Identifiants
pubmed: 37588178
doi: 10.1016/j.bjao.2023.100143
pii: S2772-6096(23)00022-9
pmc: PMC10430808
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100143Informations de copyright
© 2023 The Author(s).
Références
Mol Brain. 2013 Aug 22;6:38
pubmed: 23965342
Anesth Analg. 2023 May 1;136(5):999-1011
pubmed: 36469752
Nat Rev Neurosci. 2009 May;10(5):373-83
pubmed: 19377502
Cell Rep. 2018 Oct 23;25(4):974-987.e4
pubmed: 30355502
Front Cell Dev Biol. 2021 Jul 07;9:684516
pubmed: 34307363
J Clin Invest. 2014 Dec;124(12):5437-41
pubmed: 25365226
J Neurosci Methods. 2000 Jul 31;100(1-2):117-22
pubmed: 11040373
Brain Res. 2006 Dec 6;1123(1):80-8
pubmed: 17049496
Methods Mol Biol. 2014;1183:221-42
pubmed: 25023312
Molecules. 2022 Aug 26;27(17):
pubmed: 36080253
Anesthesiology. 2008 Jan;108(1):18-30
pubmed: 18156878
J Neuroinflammation. 2015 Jun 06;12:114
pubmed: 26048578
Crit Care. 2010;14(3):R88
pubmed: 20470406
Br J Anaesth. 2023 Jan;130(1):e7-e10
pubmed: 36336522
PLoS One. 2013 May 28;8(5):e64732
pubmed: 23724087
eNeuro. 2022 Nov 15;9(6):
pubmed: 36265903
Crit Care Med. 2020 Apr;48(4):533-544
pubmed: 32205600
Antioxid Redox Signal. 2011 May 15;14(10):2013-54
pubmed: 20649473
Neurosci Bull. 2015 Apr;31(2):265-70
pubmed: 25648546
Curr Opin Neurobiol. 2022 Aug;75:102553
pubmed: 35594578
Nat Rev Neurosci. 2016 Oct 18;17(11):705-717
pubmed: 27752068
Br J Anaesth. 2018 Nov;121(5):1005-1012
pubmed: 30336844
Nat Immunol. 2020 Nov;21(11):1319-1326
pubmed: 33077953
Br J Anaesth. 2023 Feb;130(2):e351-e360
pubmed: 36402576
Curr Opin Neurobiol. 2017 Apr;43:87-93
pubmed: 28236778
Br J Anaesth. 2019 Oct;123(4):464-478
pubmed: 31439308
PLoS One. 2011;6(5):e20676
pubmed: 21655192
Cell Rep. 2012 Sep 27;2(3):488-96
pubmed: 22999935
J Neurosci. 2007 Dec 26;27(52):14326-37
pubmed: 18160640
Br J Anaesth. 2018 Sep;121(3):595-604
pubmed: 30115258
Nat Neurosci. 2022 Feb;25(2):180-190
pubmed: 35087246
Ann Surg. 2023 Mar 1;277(3):e689-e698
pubmed: 34225294
J Neurophysiol. 2021 Jul 1;126(1):11-27
pubmed: 34038186
Trends Neurosci. 2022 Dec;45(12):884-898
pubmed: 36404455
Br J Anaesth. 2022 Oct;129(4):555-566
pubmed: 35701270
Proc Natl Acad Sci U S A. 2019 Feb 12;116(7):2701-2706
pubmed: 30692251
PLoS One. 2017 Aug 7;12(8):e0182471
pubmed: 28787017
Anesthesiology. 2020 Jan;132(1):55-68
pubmed: 31834869
EBioMedicine. 2018 Nov;37:547-556
pubmed: 30348620
J Ethnopharmacol. 2021 Jul 15;275:114164
pubmed: 33932516
Transl Psychiatry. 2020 Jun 9;10(1):186
pubmed: 32518376
Cell Rep. 2021 Nov 9;37(6):109960
pubmed: 34758303