Single-molecule analysis of DNA base-stacking energetics using patterned DNA nanostructures.
Journal
Nature nanotechnology
ISSN: 1748-3395
Titre abrégé: Nat Nanotechnol
Pays: England
ID NLM: 101283273
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
13
12
2022
accepted:
10
07
2023
pubmed:
18
8
2023
medline:
18
8
2023
entrez:
17
8
2023
Statut:
ppublish
Résumé
The DNA double helix structure is stabilized by base-pairing and base-stacking interactions. However, a comprehensive understanding of dinucleotide base-stacking energetics is lacking. Here we combined multiplexed DNA-based point accumulation in nanoscale topography (DNA-PAINT) imaging with designer DNA nanostructures and measured the free energy of dinucleotide base stacking at the single-molecule level. Multiplexed imaging enabled us to extract the binding kinetics of an imager strand with and without additional dinucleotide stacking interactions. The DNA-PAINT data showed that a single additional dinucleotide base stacking results in up to 250-fold stabilization for the DNA duplex nanostructure. We found that the dinucleotide base-stacking energies vary from -0.95 ± 0.12 kcal mol
Identifiants
pubmed: 37591937
doi: 10.1038/s41565-023-01485-1
pii: 10.1038/s41565-023-01485-1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1474-1482Subventions
Organisme : DST | Science and Engineering Research Board (SERB)
ID : SRG-2021-0001553
Organisme : Department of Biotechnology, Ministry of Science and Technology (DBT)
ID : BT/PR27952/IN/22/212/2018
Organisme : Department of Biotechnology, Ministry of Science and Technology (DBT)
ID : BT/PR40186/BTIS/137/3/2020
Organisme : DBT India Alliance (Wellcome Trust/DBT India Alliance)
ID : IA/I/21/2/505928
Informations de copyright
© 2023. The Author(s).
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