Oral microbiome homogeneity across diverse human groups from southern Africa: first results from southwestern Angola and Zimbabwe.

African populations Exome sequencing Metagenomics Oral microbiome Oral microbiota Saliva Socio-economic status Subsistence methods

Journal

BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981

Informations de publication

Date de publication:
18 08 2023
Historique:
received: 31 03 2023
accepted: 04 08 2023
medline: 21 8 2023
pubmed: 19 8 2023
entrez: 18 8 2023
Statut: epublish

Résumé

While the human oral microbiome is known to play an important role in systemic health, its average composition and diversity patterns are still poorly understood. To gain better insights into the general composition of the microbiome on a global scale, the characterization of microbiomes from a broad range of populations, including non-industrialized societies, is needed. Here, we used the portion of non-human reads obtained through an expanded exome capture sequencing approach to characterize the saliva microbiomes of 52 individuals from eight ethnolinguistically diverse southern African populations from Angola (Kuvale, Kwepe, Himba, Tjimba, Kwisi, Twa, !Xun) and Zimbabwe (Tshwa), including foragers, food-producers, and peripatetic groups (low-status communities who provide services to their dominant neighbors). Our results indicate that neither host genetics nor livelihood seem to influence the oral microbiome profile, with Neisseria, Streptococcus, Prevotella, Rothia, and Porphyromonas being the five most frequent genera in southern African groups, in line with what has been shown for other human populations. However, we found that some Tshwa and Twa individuals display an enrichment of pathogenic genera from the Enterobacteriaceae family (i.e. Enterobacter, Citrobacter, Salmonella) of the Proteobacteria phylum, probably reflecting deficient sanitation and poor health conditions associated with social marginalization. Taken together, our results suggest that socio-economic status, rather than ethnolinguistic affiliation or subsistence mode, is a key factor in shaping the salivary microbial profiles of human populations in southern Africa.

Sections du résumé

BACKGROUND
While the human oral microbiome is known to play an important role in systemic health, its average composition and diversity patterns are still poorly understood. To gain better insights into the general composition of the microbiome on a global scale, the characterization of microbiomes from a broad range of populations, including non-industrialized societies, is needed. Here, we used the portion of non-human reads obtained through an expanded exome capture sequencing approach to characterize the saliva microbiomes of 52 individuals from eight ethnolinguistically diverse southern African populations from Angola (Kuvale, Kwepe, Himba, Tjimba, Kwisi, Twa, !Xun) and Zimbabwe (Tshwa), including foragers, food-producers, and peripatetic groups (low-status communities who provide services to their dominant neighbors).
RESULTS
Our results indicate that neither host genetics nor livelihood seem to influence the oral microbiome profile, with Neisseria, Streptococcus, Prevotella, Rothia, and Porphyromonas being the five most frequent genera in southern African groups, in line with what has been shown for other human populations. However, we found that some Tshwa and Twa individuals display an enrichment of pathogenic genera from the Enterobacteriaceae family (i.e. Enterobacter, Citrobacter, Salmonella) of the Proteobacteria phylum, probably reflecting deficient sanitation and poor health conditions associated with social marginalization.
CONCLUSIONS
Taken together, our results suggest that socio-economic status, rather than ethnolinguistic affiliation or subsistence mode, is a key factor in shaping the salivary microbial profiles of human populations in southern Africa.

Identifiants

pubmed: 37596536
doi: 10.1186/s12866-023-02970-2
pii: 10.1186/s12866-023-02970-2
pmc: PMC10436416
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

226

Informations de copyright

© 2023. BioMed Central Ltd., part of Springer Nature.

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Auteurs

Vítor Araújo (V)

Centro de Investigação em Biodiversidade e Recursos Genéticos, CIBIO, InBIO Laboratório Associado, Universidade do Porto, Campus de Vairão, Vairão, 4485-661, Portugal.
Program in Genomics, Biodiversity and Land Planning, CIBIO, BIOPOLIS, Campus de Vairão, Vairão, 4485-661, Portugal.
Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Porto, 4169-007, Portugal.

Anne-Maria Fehn (AM)

Centro de Investigação em Biodiversidade e Recursos Genéticos, CIBIO, InBIO Laboratório Associado, Universidade do Porto, Campus de Vairão, Vairão, 4485-661, Portugal.
Program in Genomics, Biodiversity and Land Planning, CIBIO, BIOPOLIS, Campus de Vairão, Vairão, 4485-661, Portugal.

Admire Phiri (A)

Department of Linguistics and Language Practice, University of Free State, Bloemfontein, South Africa.

Jeffrey Wills (J)

University of Zimbabwe, Harare, Zimbabwe.

Jorge Rocha (J)

Centro de Investigação em Biodiversidade e Recursos Genéticos, CIBIO, InBIO Laboratório Associado, Universidade do Porto, Campus de Vairão, Vairão, 4485-661, Portugal.
Program in Genomics, Biodiversity and Land Planning, CIBIO, BIOPOLIS, Campus de Vairão, Vairão, 4485-661, Portugal.
Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Porto, 4169-007, Portugal.

Magdalena Gayà-Vidal (M)

Centro de Investigação em Biodiversidade e Recursos Genéticos, CIBIO, InBIO Laboratório Associado, Universidade do Porto, Campus de Vairão, Vairão, 4485-661, Portugal. magdagaya@gmail.com.
Program in Genomics, Biodiversity and Land Planning, CIBIO, BIOPOLIS, Campus de Vairão, Vairão, 4485-661, Portugal. magdagaya@gmail.com.

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