GR5 acts in the G protein pathway to regulate grain size in rice.

AP2 transcription factor G protein γ subunit GR5 grain size rice (Oryza sativa L.)

Journal

Plant communications
ISSN: 2590-3462
Titre abrégé: Plant Commun
Pays: China
ID NLM: 101769147

Informations de publication

Date de publication:
18 Aug 2023
Historique:
received: 24 02 2023
revised: 09 08 2023
accepted: 14 08 2023
pubmed: 19 8 2023
medline: 19 8 2023
entrez: 19 8 2023
Statut: aheadofprint

Résumé

Grain size is an important determinant of grain yield in rice. Although dozens of grain size genes have been reported, the molecular mechanisms that control grain size remain to be fully clarified. Here, we report the cloning and characterization of GR5 (GRAIN ROUND 5), which is allelic to SMOS1/SHB/RLA1/NGR5 and encodes an AP2 transcription factor. GR5 acts as a transcriptional activator and determines grain size by influencing cell proliferation and expansion. We demonstrated that GR5 physically interacts with five Gγ subunit proteins (RGG1, RGG2, DEP1, GS3, and GGC2) and acts downstream of the G protein complex. Four downstream target genes of GR5 in grain development (DEP2, DEP3, DRW1, and CyCD5;2) were revealed and their core T/CGCAC motif identified by yeast one-hybrid, EMSA, and ChIP-PCR experiments. Our results revealed that GR5 interacts with Gγ subunits and cooperatively determines grain size by regulating the expression of downstream target genes. These findings provide new insight into the genetic regulatory network of the G protein signaling pathway in the control of grain size and provide a potential target for high-yield rice breeding.

Identifiants

DOI: 10.1016/j.xplc.2023.100673 PMID: 37596786
pubmed: 37596786
pii: S2590-3462(23)00201-8
doi: 10.1016/j.xplc.2023.100673
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

100673

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Yueying Wang (Y)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Yang Lv (Y)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Haiping Yu (H)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Peng Hu (P)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Yi Wen (Y)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Junge Wang (J)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Yiqing Tan (Y)

Nanjing Agricultural University, Nan Jing 210000, Jiangsu, China.

Hao Wu (H)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Lixin Zhu (L)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Kaixiong Wu (K)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Bingze Chai (B)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Jialong Liu (J)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Dali Zeng (D)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Guangheng Zhang (G)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Li Zhu (L)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Zhenyu Gao (Z)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Guojun Dong (G)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Deyong Ren (D)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Lan Shen (L)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Qiang Zhang (Q)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Qing Li (Q)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Longbiao Guo (L)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Guosheng Xiong (G)

Nanjing Agricultural University, Nan Jing 210000, Jiangsu, China. Electronic address: gsxiong@njau.edu.cn.

Qian Qian (Q)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Hainan Yazhou Bay Seed Laboratory, Sanya 572024, Hainan, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China. Electronic address: qianiqn188@hotmail.com.

Jiang Hu (J)

State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Hainan Yazhou Bay Seed Laboratory, Sanya 572024, Hainan, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China. Electronic address: hujiang588@163.com.

Classifications MeSH