Physicochemical and biological analysis of composite biomaterials containing hydroxyapatite for biological applications.
cytotoxicity
hydroxyapatite
polymer-ceramic composites
sorption ability
wound healing assay
Journal
Journal of biomedical materials research. Part B, Applied biomaterials
ISSN: 1552-4981
Titre abrégé: J Biomed Mater Res B Appl Biomater
Pays: United States
ID NLM: 101234238
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
11
06
2023
received:
09
02
2023
accepted:
31
07
2023
pubmed:
19
8
2023
medline:
19
8
2023
entrez:
19
8
2023
Statut:
ppublish
Résumé
Bone tissue regeneration is one of the main areas of tissue engineering. A particularly important aspect is the development of new innovative composite materials intended for bone tissue engineering and/or bone substitution. In this article, the synthesis and characterization of ceramic-polymer composites based on polyvinylpyrrolidone, poly(vinyl alcohol) and hydroxyapatite (HAp) have been presented. The first part of the work deals with the synthesis and characterization of the ceramic phase. It was demonstrated that the obtained calcium phosphate is characterized by a heterogeneity and porosity indicating simultaneously its large specific surface area. Additionally, in the wound healing test, it was shown that the obtained powder supports the regeneration of L929 cells. Next, HAp-containing composite materials were obtained in the waste-free photopolymerization process and characterized in detail. It was proved that the obtained composites were characterized by sorption properties and stability during 12-day incubation in simulated physiological liquids. Importantly, the obtained composites showed no cytotoxic effect against the L929 murine fibroblasts - the cell viability was 94.5%. Then, confocal microscopy allowed to observe that murine fibroblasts effectively colonized the surface of the obtained polymer-ceramic composites, covering the entire surface of the biomaterial. Thus, the obtained results confirm the high potential of the obtained composites in the application of bone tissue regenerative medicine.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2077-2088Subventions
Organisme : Foundation for Polish Science
ID : POIR.04.04.00-00-16D7/18
Organisme : TEAM-NET program of the Foundation for Polish Science
Organisme : European Union under the European Regional Development Fund
Informations de copyright
© 2023 Wiley Periodicals LLC.
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