Z-Form Extracellular DNA in Pediatric CRS May Provide a Mechanism for Recalcitrance to Treatment.
Biofilm
DNABII Proteins
FESS
PMNs
Journal
The Laryngoscope
ISSN: 1531-4995
Titre abrégé: Laryngoscope
Pays: United States
ID NLM: 8607378
Informations de publication
Date de publication:
Apr 2024
Apr 2024
Historique:
revised:
21
07
2023
received:
19
05
2023
accepted:
09
08
2023
pmc-release:
19
02
2025
medline:
18
3
2024
pubmed:
19
8
2023
entrez:
19
8
2023
Statut:
ppublish
Résumé
We examined sinus mucosal samples recovered from pediatric chronic rhinosinusitis (CRS) patients for the presence of Z-form extracellular DNA (eDNA) due to its recently elucidated role in pathogenesis of disease. Further, we immunolabeled these specimens for the presence of both members of the bacterial DNA-binding DNABII protein family, integration host factor (IHF) and histone-like protein (HU), due to their known role in converting common B-DNA to the rare Z-form. Sinus mucosa samples recovered from 20 patients during functional endoscopic sinus surgery (FESS) were immunolabelled for B- and Z-DNA, as well as for both bacterial DNABII proteins. Nineteen of 20 samples (95%) included areas rich in eDNA, with the majority in the Z-form. Areas positive for B-DNA were restricted to the most distal regions of the mucosal specimen. Labeling for both DNABII proteins was observed on B- and Z-DNA, which aligned with the role of these proteins in the B-to-Z DNA conversion. Abundant Z-form eDNA in culture-positive pediatric CRS samples suggested that bacterial DNABII proteins were responsible for the conversion of eukaryotic B-DNA that had been released into the luminal space by PMNs during NETosis, to the Z-form. The presence of both DNABII proteins on B-DNA and Z-DNA supported the known role of these bacterial proteins in the B-to-Z DNA conversion. Given that Z-form DNA both stabilizes the bacterial biofilm and inactivates PMN NET-mediated killing of trapped bacteria, we hypothesize that this conversion may be contributing to the chronicity and recalcitrance of CRS to treatment. NA Laryngoscope, 134:1564-1571, 2024.
Identifiants
pubmed: 37597166
doi: 10.1002/lary.30986
pmc: PMC10875147
mid: NIHMS1932891
doi:
Substances chimiques
DNA, Z-Form
0
DNA, B-Form
0
Integration Host Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1564-1571Subventions
Organisme : NIDCD NIH HHS
ID : R01 DC003915
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC011818
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC003915
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC011818
Pays : United States
Informations de copyright
© 2023 The American Laryngological, Rhinological and Otological Society, Inc.
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