Computationally-driven epitope identification of PEDV N-protein and its application in development of immunoassay for PEDV detection.
Epitopes of N protein
Immunochromatographic assay
Monoclonal antibody
Porcine epidemic diarrhea virus
Quantum dots
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
25 Oct 2023
25 Oct 2023
Historique:
received:
15
06
2023
revised:
16
08
2023
accepted:
16
08
2023
medline:
11
9
2023
pubmed:
21
8
2023
entrez:
20
8
2023
Statut:
ppublish
Résumé
The nucleocapsid (N) protein is a suitable candidate for early diagnosis of porcine epidemic diarrhea virus (PEDV). Here, we identified the linear B-cell epitopes of the PEDV N-protein by integrating a computational-experimental framework and constructed three-dimensional (3D) structure model of the N protein using the ColabFold program in Google Colaboratory. Furthermore, we prepared the monoclonal antibodies against the predicted epitopes and recombinant N protein, respectively, and selected pairing mAbs (named 9C4 and 3C5) to develop a double-antibody sandwich immunochromatographic test strip using CdSe/ZnS quantum dots (QDs)-labelled 9C4 and 3C5 as capture and detection antibodies, respectively. This strip can specifically detect PEDV within 10 min with a detection limit of less than 6.25 × 10
Identifiants
pubmed: 37598469
pii: S0731-7085(23)00429-6
doi: 10.1016/j.jpba.2023.115660
pii:
doi:
Substances chimiques
Epitopes
0
Antibodies, Monoclonal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115660Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.