Single-cell scattering and auto-fluorescence-based fast antibiotic susceptibility testing for gram-negative and gram-positive bacteria.
Escherichia coli
Staphylococcus epidermidis
aminoglycoside
antibiotic susceptibility testing
beta-lactam
flow cytometry
growth-free
label-free
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2023
2023
Historique:
received:
31
05
2023
accepted:
20
07
2023
medline:
21
8
2023
pubmed:
21
8
2023
entrez:
21
8
2023
Statut:
epublish
Résumé
In this study, we assess the scattering of light and auto-fluorescence from single bacterial cells to address the challenge of fast (<2 h), label-free phenotypic antimicrobial susceptibility testing (AST). Label-free flow cytometry is used for monitoring both the respiration-related auto-fluorescence in two different fluorescence channels corresponding to FAD and NADH, and the morphological and structural information contained in the light scattered by individual bacteria during incubation with or without antibiotic. Large multi-parameter data are analyzed using dimensionality reduction methods, based either on a combination of 2D binning and Principal Component Analysis, or with a one-class Support Vector Machine approach, with the objective to predict the Susceptible or Resistant phenotype of the strain. For the first time, both
Identifiants
pubmed: 37601345
doi: 10.3389/fmicb.2023.1232250
pmc: PMC10436599
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1232250Informations de copyright
Copyright © 2023 Dixneuf, Chareire-Kleiberg, Mahé, El Azami, Kolytcheff, Bellais, Guyard, Védrine, Mallard, Josso and Rol.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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