Investigation of three common centrifugation protocols for platelet-rich fibrin (PRF) as a bio-carrier for ampicillin/sulbactam: a prospective trial.
Agar diffusion test
Ampicillin
Antibiotics
Bio-carrier
Centrifugation
Local antibiotic application
PRF
Platelet-rich fibrin
Protocol
Sulbactam
Journal
Clinical oral investigations
ISSN: 1436-3771
Titre abrégé: Clin Oral Investig
Pays: Germany
ID NLM: 9707115
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
16
03
2023
accepted:
14
08
2023
medline:
9
10
2023
pubmed:
21
8
2023
entrez:
21
8
2023
Statut:
ppublish
Résumé
Different platelet-rich fibrin (PRF) protocols exist and are known to differ in resulting mechanical and bioactive properties. Centrifugation parameters may also influence drug release, in particular antibiotics, when using PRF as a bio-carrier. We thus evaluated three common protocols regarding effects on the bio-carrier properties. In a prospective trial comprising 33 patients, we compared different protocols for PRF as a bio-carrier for ampicillin/sulbactam (SAM). Blood samples were taken shortly after a single dose of ampicillin/sulbactam (2 g/1 g) was administered to patients intravenously. PRF was obtained by centrifugation and three protocols were used: protocol A (1300 rpm, 8 min, RCF-max = 208 g), B (2300 rpm, 12 min, RCF-max = 652 g), and C (1500 rpm, 14 min, RCF-max = 276 g). The antibacterial activity of PRF was investigated against five oral species in vitro, based on agar diffusion methodology. The study demonstrates that a single dose of SAM is sufficient to reach high concentrations in PRF in all protocols (150 µg/ml), which is comparable to the plasma SAM concentration. Antibacterial activity was inferred from the diameter of inhibition zones seen in agar diffusion tests using PRF discs. Protocol B resulted in the largest inhibition zones. One-way ANOVA revealed statistically improved results for protocol B for some bacteria. The study provides valuable data on PRF antibiotic enrichment, notably SAM. A single dose of SAM is sufficient to reach clinically relevant concentrations in PRF. These findings potentially extend the application of PRF, for example in patients with osteonecrosis of the jaw or in oral surgery (e.g., stick bone).
Identifiants
pubmed: 37603167
doi: 10.1007/s00784-023-05212-x
pii: 10.1007/s00784-023-05212-x
pmc: PMC10560174
doi:
Substances chimiques
sultamicillin
65DT0ML581
Sulbactam
S4TF6I2330
Agar
9002-18-0
Intercellular Signaling Peptides and Proteins
0
Anti-Bacterial Agents
0
Ampicillin
7C782967RD
Types de publication
Journal Article
Langues
eng
Pagination
5991-5998Informations de copyright
© 2023. The Author(s).
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