Risk assessment of Enterococcus faecium, Klebsiella pneumoniae, and Pseudomonas aeruginosa in environmental water sources: Development of surrogate models for antibiotic resistance genes.

E. faecium K. pneumoniae P. aeruginosa Risk assessment aac(6′)-Ib gene aac(6′)-aph(2″) gene

Journal

The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500

Informations de publication

Date de publication:
25 Nov 2023
Historique:
received: 31 03 2023
revised: 08 08 2023
accepted: 08 08 2023
pubmed: 22 8 2023
medline: 22 8 2023
entrez: 21 8 2023
Statut: ppublish

Résumé

The presence of Enterococcus faecium (E. faecium), Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa), and the aminoglycoside resistance genes, aac(6')-Ib and aac(6')-aph(2″), was investigated in environmental water sources obtained from informal settlements in the Western Cape (South Africa). Using ethidium monoazide bromide quantitative polymerase chain reaction (EMA-qPCR) analysis, E. faecium, K. pneumoniae, and P. aeruginosa were detected in 88.9 %, 100 %, and 93.3 % of the samples (n = 45), respectively, with a significantly higher mean concentration recorded for K. pneumoniae (7.83 × 10

Identifiants

pubmed: 37604372
pii: S0048-9697(23)04842-8
doi: 10.1016/j.scitotenv.2023.166217
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

166217

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Wesaal Khan reports financial support was provided by Water Research Commission. Wesaal Khan reports financial support was provided by National Research Foundation.

Auteurs

Julia Denissen (J)

Department of Microbiology, Faculty of Science, Stellenbosch University, Private Bag X1, Stellenbosch 7602, South Africa.

Brandon Reyneke (B)

Department of Microbiology, Faculty of Science, Stellenbosch University, Private Bag X1, Stellenbosch 7602, South Africa.

Tobias Barnard (T)

Water and Health Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein 7305, South Africa.

Sehaam Khan (S)

Water and Health Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein 7305, South Africa.

Wesaal Khan (W)

Department of Microbiology, Faculty of Science, Stellenbosch University, Private Bag X1, Stellenbosch 7602, South Africa. Electronic address: wesaal@sun.ac.za.

Classifications MeSH