Immunological profile of mice immunized with a polyvalent virosome-based influenza vaccine.


Journal

Virology journal
ISSN: 1743-422X
Titre abrégé: Virol J
Pays: England
ID NLM: 101231645

Informations de publication

Date de publication:
21 08 2023
Historique:
received: 24 05 2023
accepted: 11 08 2023
medline: 23 8 2023
pubmed: 22 8 2023
entrez: 22 8 2023
Statut: epublish

Résumé

Influenza A virus (IAV) causes respiratory disease in pigs and is a major concern for public health. Vaccination of pigs is the most successful measure to mitigate the impact of the disease in the herds. Influenza-based virosome is an effective immunomodulating carrier that replicates the natural antigen presentation pathway and has tolerability profile due to their purity and biocompatibility. This study aimed to develop a polyvalent virosome influenza vaccine containing the hemagglutinin and neuraminidase proteins derived from the swine IAVs (swIAVs) H1N1, H1N2 and H3N2 subtypes, and to investigate its effectiveness in mice as a potential vaccine for swine. Mice were immunized with two vaccine doses (1 and 15 days), intramuscularly and intranasally. At 21 days and eight months later after the second vaccine dose, mice were euthanized. The humoral and cellular immune responses in mice vaccinated intranasally or intramuscularly with a polyvalent influenza virosomal vaccine were investigated. Only intramuscular vaccination induced high hemagglutination inhibition (HI) titers. Seroconversion and seroprotection (> 4-fold rise in HI antibody titers, reaching a titer of ≥ 1:40) were achieved in 80% of mice (intramuscularly vaccinated group) at 21 days after booster immunization. Virus-neutralizing antibody titers against IAV were detected at 8 months after vaccination, indicating long-lasting immunity. Overall, mice immunized with the virosome displayed greater ability for B, effector-T and memory-T cells from the spleen to respond to H1N1, H1N2 and H3N2 antigens. All findings showed an efficient immune response against IAVs in mice vaccinated with a polyvalent virosome-based influenza vaccine.

Sections du résumé

BACKGROUND
Influenza A virus (IAV) causes respiratory disease in pigs and is a major concern for public health. Vaccination of pigs is the most successful measure to mitigate the impact of the disease in the herds. Influenza-based virosome is an effective immunomodulating carrier that replicates the natural antigen presentation pathway and has tolerability profile due to their purity and biocompatibility.
METHODS
This study aimed to develop a polyvalent virosome influenza vaccine containing the hemagglutinin and neuraminidase proteins derived from the swine IAVs (swIAVs) H1N1, H1N2 and H3N2 subtypes, and to investigate its effectiveness in mice as a potential vaccine for swine. Mice were immunized with two vaccine doses (1 and 15 days), intramuscularly and intranasally. At 21 days and eight months later after the second vaccine dose, mice were euthanized. The humoral and cellular immune responses in mice vaccinated intranasally or intramuscularly with a polyvalent influenza virosomal vaccine were investigated.
RESULTS
Only intramuscular vaccination induced high hemagglutination inhibition (HI) titers. Seroconversion and seroprotection (> 4-fold rise in HI antibody titers, reaching a titer of ≥ 1:40) were achieved in 80% of mice (intramuscularly vaccinated group) at 21 days after booster immunization. Virus-neutralizing antibody titers against IAV were detected at 8 months after vaccination, indicating long-lasting immunity. Overall, mice immunized with the virosome displayed greater ability for B, effector-T and memory-T cells from the spleen to respond to H1N1, H1N2 and H3N2 antigens.
CONCLUSIONS
All findings showed an efficient immune response against IAVs in mice vaccinated with a polyvalent virosome-based influenza vaccine.

Identifiants

pubmed: 37605141
doi: 10.1186/s12985-023-02158-0
pii: 10.1186/s12985-023-02158-0
pmc: PMC10463652
doi:

Substances chimiques

Influenza Vaccines 0
Vaccines, Combined 0
Vaccines, Virosome 0
Virosomes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

187

Informations de copyright

© 2023. BioMed Central Ltd., part of Springer Nature.

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Auteurs

Francisco Noé Fonseca (FN)

Embrapa Suínos e Aves, Concórdia, SC, Brazil.

Vanessa Haach (V)

Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Franciana Volpato Bellaver (FV)

Instituto Federal Catarinense - Campus Concórdia, Concórdia, SC, Brazil.

Gabrielly Bombassaro (G)

Instituto Federal Catarinense - Campus Concórdia, Concórdia, SC, Brazil.

Danielle Gava (D)

Embrapa Suínos e Aves, Concórdia, SC, Brazil.

Luciano Paulino da Silva (LP)

Embrapa Recursos Genéticos, Brasília, DF, Brazil.

Lana Flavia Baron (LF)

Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Mayara Simonelly (M)

Universidade de Brasília, Brasília, DF, Brazil.

Wanessa Araújo Carvalho (WA)

Embrapa Gado de Leite, Juiz de Fora-MG, Juiz de Fora, MG, Brazil.

Rejane Schaefer (R)

Embrapa Suínos e Aves, Concórdia, SC, Brazil.

Ana Paula Bastos (AP)

Embrapa Suínos e Aves, Concórdia, SC, Brazil. ana.bastos@embrapa.br.

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