Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouse.

5xFAD Acid sphingomyelinase Astrocytes C1q Extracellular vesicle IL-1α Imipramine Microglia Mitochondria TNF-α

Journal

Acta neuropathologica communications
ISSN: 2051-5960
Titre abrégé: Acta Neuropathol Commun
Pays: England
ID NLM: 101610673

Informations de publication

Date de publication:
21 08 2023
Historique:
received: 15 06 2023
accepted: 05 08 2023
medline: 23 8 2023
pubmed: 22 8 2023
entrez: 22 8 2023
Statut: epublish

Résumé

In Alzheimer's disease (AD), reactive astrocytes produce extracellular vesicles (EVs) that affect mitochondria in neurons. Here, we show that Aβ-induced generation of the sphingolipid ceramide by acid sphingomyelinase (A-SMase) triggered proinflammatory cytokine (C1q, TNF-α, IL-1α) release by microglia, which induced the reactive astrocytes phenotype and secretion of EVs enriched with ceramide. These EVs impeded the capacity of neurons to respond to energy demand. Inhibition of A-SMase with Arc39 and Imipramine reduced the secretion of cytokines from microglia, prompting us to test the effect of Imipramine on EV secretion and AD pathology in the 5xFAD mouse model. Brain derived-EVs from 5xFAD mice treated with Imipramine contained reduced levels of the astrocytic marker GFAP, ceramide, and Aβ and did not impair mitochondrial respiration when compared to EVs derived from untreated 5xFAD brain. Consistently, Imipramine-treated 5xFAD mice showed reduced AD pathology. Our study identifies A-SMase inhibitors as potential AD therapy by preventing cyotokine-elicited secretion of mitotoxic EVs from astrocytes.

Identifiants

pubmed: 37605262
doi: 10.1186/s40478-023-01633-7
pii: 10.1186/s40478-023-01633-7
pmc: PMC10440899
doi:

Substances chimiques

Sphingomyelin Phosphodiesterase EC 3.1.4.12
Imipramine OGG85SX4E4
Ceramides 0

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

135

Subventions

Organisme : NIA NIH HHS
ID : R21 AG078601
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG064234
Pays : United States
Organisme : NIA NIH HHS
ID : P30 AG028383
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG034389
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG078338
Pays : United States
Organisme : BLRD VA
ID : I01 BX003643
Pays : United States

Informations de copyright

© 2023. BioMed Central Ltd., part of Springer Nature.

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Auteurs

Simone M Crivelli (SM)

Department of Physiology, University of Kentucky College of Medicine, 780 Rose Street MS519, Lexington, KY, 40536, USA.

Zainuddin Quadri (Z)

Department of Physiology, University of Kentucky College of Medicine, 780 Rose Street MS519, Lexington, KY, 40536, USA.

Hemendra J Vekaria (HJ)

Department of Neuroscience, University of Kentucky, Lexington, KY, 40536, USA.
Spinal Cord and Brain Injury Research Center (SCoBIRC), University of Kentucky, Lexington, KY, USA.
Veterans Affairs Medical Center, Lexington, KY, 40502, USA.

Zhihui Zhu (Z)

Department of Physiology, University of Kentucky College of Medicine, 780 Rose Street MS519, Lexington, KY, 40536, USA.

Priyanka Tripathi (P)

Department of Physiology, University of Kentucky College of Medicine, 780 Rose Street MS519, Lexington, KY, 40536, USA.

Ahmed Elsherbini (A)

Department of Physiology, University of Kentucky College of Medicine, 780 Rose Street MS519, Lexington, KY, 40536, USA.

Liping Zhang (L)

Department of Physiology, University of Kentucky College of Medicine, 780 Rose Street MS519, Lexington, KY, 40536, USA.

Patrick G Sullivan (PG)

Department of Neuroscience, University of Kentucky, Lexington, KY, 40536, USA.
Spinal Cord and Brain Injury Research Center (SCoBIRC), University of Kentucky, Lexington, KY, USA.
Veterans Affairs Medical Center, Lexington, KY, 40502, USA.

Erhard Bieberich (E)

Department of Physiology, University of Kentucky College of Medicine, 780 Rose Street MS519, Lexington, KY, 40536, USA. erhard.bieberich@uky.edu.
Veterans Affairs Medical Center, Lexington, KY, 40502, USA. erhard.bieberich@uky.edu.

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