Molecular insights using spatial transcriptomics of the distal lung in congenital diaphragmatic hernia.
Infant, Newborn
Humans
Male
Female
Hernias, Diaphragmatic, Congenital
/ genetics
Transcriptome
/ genetics
Endothelial Cells
/ metabolism
Lung
/ metabolism
Hypertension, Pulmonary
/ metabolism
Phenyl Ethers
/ metabolism
Muscle Proteins
/ metabolism
Intracellular Signaling Peptides and Proteins
/ metabolism
LIM Domain Proteins
/ metabolism
congenital diaphragmatic hernia
pulmonary hypertension
spatial transcriptomics
Journal
American journal of physiology. Lung cellular and molecular physiology
ISSN: 1522-1504
Titre abrégé: Am J Physiol Lung Cell Mol Physiol
Pays: United States
ID NLM: 100901229
Informations de publication
Date de publication:
01 10 2023
01 10 2023
Historique:
pmc-release:
01
10
2024
medline:
5
10
2023
pubmed:
22
8
2023
entrez:
22
8
2023
Statut:
ppublish
Résumé
Abnormal pulmonary vascular development and function in congenital diaphragmatic hernia (CDH) is a significant factor leading to pulmonary hypertension. The lung is a very heterogenous organ and has marked cellular diversity that is differentially responsive to injury and therapeutic agents. Spatial transcriptomics provides the unmatched capability of discerning the differences in the transcriptional signature of these distinct cell subpopulations in the lung with regional specificity. We hypothesized that the distal lung parenchyma (selected as a region of interest) would show a distinct transcriptomic profile in the CDH lung compared with control (normal lung). We subjected lung sections obtained from male and female CDH and control neonates to spatial transcriptomics using the Nanostring GeoMx platform. Spatial transcriptomic analysis of the human CDH and control lung revealed key differences in the gene expression signature. Increased expression of alveolar epithelial-related genes (
Identifiants
pubmed: 37605849
doi: 10.1152/ajplung.00154.2023
pmc: PMC10639013
doi:
Substances chimiques
Phenyl Ethers
0
FHL1 protein, human
0
Muscle Proteins
0
Intracellular Signaling Peptides and Proteins
0
LIM Domain Proteins
0
Banques de données
figshare
['10.6084/m9.figshare.22822355.v1']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
L477-L486Subventions
Organisme : NICHD NIH HHS
ID : R21 HD100862
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL144775
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL146395
Pays : United States
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