A Revised Diagnostic Quantitative RT-PCR for the Detection of Nipah Virus Infection.
Nipah virus
Nucleoprotein gene
Primer
Probe
Quantitative RT-PCR
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
24
8
2023
pubmed:
23
8
2023
entrez:
23
8
2023
Statut:
ppublish
Résumé
From its discovery in Malaysia in the late 1990s, the spillover of the Nipah virus from its pteropid reservoir into the human population has resulted in sporadic outbreaks of fatal encephalitis and respiratory disease. In this chapter, we revise a previously described quantitative reverse transcription polymerase chain reaction method, which now utilizes degenerate nucleotides at certain positions in the probe and the reverse primer to accommodate the sequence heterogeneity observed within the Nipah henipavirus species.
Identifiants
pubmed: 37610571
doi: 10.1007/978-1-0716-3283-3_2
doi:
Substances chimiques
Nucleotides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25-31Informations de copyright
© 2023. Springer Science+Business Media, LLC, part of Springer Nature.
Références
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