In Vivo Imaging of Nipah Virus Infection in Small Animal Rodent Models.
Fluorescence
In vivo imaging
Luminescence
Recombinant Nipah virus
Reporter genes
Rodent animal model
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
24
8
2023
pubmed:
23
8
2023
entrez:
23
8
2023
Statut:
ppublish
Résumé
In vivo imaging system (IVIS) is a powerful tool for the study of infectious diseases, providing the ability to non-invasively follow viral infection in an individual animal over time. Recombinant henipaviruses expressing bioluminescent or fluorescent reporter proteins can be used both to monitor the spatial and temporal progression of Nipah virus (NiV) infection in vivo as well as in ex vivo tissues. Virally produced luciferases react with systemically administered substrate to produce bioluminescence that can then be detected via IVIS imaging, while fluorescent reporters inherently generate detectable fluorescence without a substrate. Here we describe protocols applying bioluminescent or fluorescent reporter expressing recombinant viruses to in vivo or ex vivo imaging of NiV infection.
Identifiants
pubmed: 37610580
doi: 10.1007/978-1-0716-3283-3_11
doi:
Substances chimiques
Coloring Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
149-157Subventions
Organisme : NIAID NIH HHS
ID : R21 AI138233
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI123449
Pays : United States
Organisme : NIAID NIH HHS
ID : R33 AI102267
Pays : United States
Informations de copyright
© 2023. Springer Science+Business Media, LLC, part of Springer Nature.
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