Exploring a novel composite method using non-contrast EUS enhanced microvascular imaging and cyst fluid analysis to differentiate pancreatic cystic lesions.


Journal

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
ISSN: 1878-3562
Titre abrégé: Dig Liver Dis
Pays: Netherlands
ID NLM: 100958385

Informations de publication

Date de publication:
11 2023
Historique:
received: 26 02 2023
revised: 07 07 2023
accepted: 03 08 2023
medline: 30 10 2023
pubmed: 24 8 2023
entrez: 23 8 2023
Statut: ppublish

Résumé

Differentiating pancreatic cystic lesions (PCLs) remains a diagnostic challenge. The use of high-definition imaging modalities which detect tumor microvasculature have been described in solid lesions. We aim to evaluate the usefulness of cystic microvasculature when used in combination with cyst fluid biochemistry to differentiate PCLs. We retrospectively analyzed 110 consecutive patients with PCLs from 2 Italian Hospitals who underwent EUS with H-Flow and EUS fine needle aspiration to obtain cystic fluid. The accuracy of fluid biomarkers was evaluated against morphological features on radiology and EUS. Gold standard for diagnosis was surgical resection. A clinical and radiological follow up was applied in those patients who were not resected because not surgical indication and no signs of malignancy were shown. Of 110 patients, 65 were diagnosed with a mucinous cyst, 41 with a non-mucinous cyst, and 4 with an undetermined cyst. Fluid analysis alone yielded 76.7% sensitivity, 56.7% specificity, 77.8 positive predictive value (PPV), 55.3 negative predictive value (NPV) and 56% accuracy in diagnosing pancreatic cysts alone. Our composite method yielded 97.3% sensitivity, 77.1% specificity, 90.1% PPV, 93.1% NPV, 73.2% accuracy. This new composite could be applied to the holistic approach of combining cyst morphology, vascularity, and fluid analysis alongside endoscopist expertise.

Sections du résumé

BACKGROUND AND AIMS
Differentiating pancreatic cystic lesions (PCLs) remains a diagnostic challenge. The use of high-definition imaging modalities which detect tumor microvasculature have been described in solid lesions. We aim to evaluate the usefulness of cystic microvasculature when used in combination with cyst fluid biochemistry to differentiate PCLs.
METHODS
We retrospectively analyzed 110 consecutive patients with PCLs from 2 Italian Hospitals who underwent EUS with H-Flow and EUS fine needle aspiration to obtain cystic fluid. The accuracy of fluid biomarkers was evaluated against morphological features on radiology and EUS. Gold standard for diagnosis was surgical resection. A clinical and radiological follow up was applied in those patients who were not resected because not surgical indication and no signs of malignancy were shown.
RESULTS
Of 110 patients, 65 were diagnosed with a mucinous cyst, 41 with a non-mucinous cyst, and 4 with an undetermined cyst. Fluid analysis alone yielded 76.7% sensitivity, 56.7% specificity, 77.8 positive predictive value (PPV), 55.3 negative predictive value (NPV) and 56% accuracy in diagnosing pancreatic cysts alone. Our composite method yielded 97.3% sensitivity, 77.1% specificity, 90.1% PPV, 93.1% NPV, 73.2% accuracy.
CONCLUSIONS
This new composite could be applied to the holistic approach of combining cyst morphology, vascularity, and fluid analysis alongside endoscopist expertise.

Identifiants

pubmed: 37612214
pii: S1590-8658(23)00848-4
doi: 10.1016/j.dld.2023.08.038
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1548-1553

Informations de copyright

Copyright © 2023. Published by Elsevier Ltd.

Déclaration de conflit d'intérêts

Conflict of interest The authors whose names are listed immediately below certify that they have NO affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

Auteurs

Silvia Carrara (S)

Humanitas Research Hospital -IRCCS-, Endoscopy Unit, Rozzano, Italy. Electronic address: silvia.carrara@humanitas.it.

Alberto Fantin (A)

Unit of Surgical Oncology of Digestive Tract, Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy.

Kareem Khalaf (K)

Division of Gastroenterology, St. Michael's Hospital, University of Toronto, Toronto, Canada.

Tommy Rizkala (T)

Humanitas University, Department of Biomedical Sciences, Pieve Emanuele, Italy.

Glenn Koleth (G)

Humanitas Research Hospital -IRCCS-, Endoscopy Unit, Rozzano, Italy.

Marta Andreozzi (M)

Humanitas Research Hospital -IRCCS-, Endoscopy Unit, Rozzano, Italy.

Marco Spadaccini (M)

Humanitas Research Hospital -IRCCS-, Endoscopy Unit, Rozzano, Italy.

Matteo Colombo (M)

Humanitas Research Hospital -IRCCS-, Endoscopy Unit, Rozzano, Italy.

Mario Gruppo (M)

Unit of Surgical Oncology of Digestive Tract, Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy.

Cristiana Bonifacio (C)

Humanitas Research Hospital -IRCCS, Department of Radiology, Rozzano, Italy.

Francesca Gavazzi (F)

Humanitas Research Hospital -IRCCS, Pancreatic Surgery Unit, Rozzano, Italy.

Giovanni Luigi Capretti (GL)

Humanitas Research Hospital -IRCCS, Pancreatic Surgery Unit, Rozzano, Italy.

Cristina Ridolfi (C)

Humanitas Research Hospital -IRCCS, Pancreatic Surgery Unit, Rozzano, Italy.

Gennaro Nappo (G)

Humanitas Research Hospital -IRCCS, Pancreatic Surgery Unit, Rozzano, Italy.

Paola Spaggiari (P)

Humanitas Research Hospital -IRCCS-, Endoscopy Unit, Rozzano, Italy.

Luca Di Tommaso (LD)

Humanitas Research Hospital -IRCCS, Pathology Unit, Rozzano, Italy.

Mauro Sollai (M)

Humanitas Research Hospital -IRCCS, Pathology Unit, Rozzano, Italy.

Alessandro Zerbi (A)

Humanitas University, Department of Biomedical Sciences, Pieve Emanuele, Italy; Humanitas Research Hospital -IRCCS, Pancreatic Surgery Unit, Rozzano, Italy.

Roberta Maselli (R)

Humanitas Research Hospital -IRCCS-, Endoscopy Unit, Rozzano, Italy; Humanitas University, Department of Biomedical Sciences, Pieve Emanuele, Italy.

Alessandro Fugazza (A)

Humanitas Research Hospital -IRCCS-, Endoscopy Unit, Rozzano, Italy.

Cesare Hassan (C)

Humanitas Research Hospital -IRCCS-, Endoscopy Unit, Rozzano, Italy; Humanitas University, Department of Biomedical Sciences, Pieve Emanuele, Italy.

Antonio Facciorusso (A)

Section of Gastroenterology, Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy.

Alessandro Repici (A)

Humanitas Research Hospital -IRCCS-, Endoscopy Unit, Rozzano, Italy; Humanitas University, Department of Biomedical Sciences, Pieve Emanuele, Italy.

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