A Transcriptomic Comparative Study of Cranial Vasculature.

Cerebral vasculature Intracerebral artery Intracranial aneurysm Superficial temporal artery

Journal

Translational stroke research

ISSN: 1868-601X

Titre abrégé: Transl Stroke Res

Pays: United States

ID NLM: 101517297

Informations de publication

Date de publication:
23 Aug 2023

Historique:

received: 06 07 2023

accepted: 07 08 2023

revised: 06 07 2023

medline: 24 8 2023

pubmed: 24 8 2023

entrez: 23 8 2023

Statut: aheadofprint

Résumé

In genetic studies of cerebrovascular diseases, the optimal vessels to use as controls remain unclear. Our goal is to compare the transcriptomic profiles among 3 different types of control vessels: superficial temporal artery (STA), middle cerebral arteries (MCA), and arteries from the circle of Willis obtained from autopsies (AU). We examined the transcriptomic profiles of STA, MCA, and AU using RNAseq. We also investigated the effects of using these control groups on the results of the comparisons between aneurysms and the control arteries. Our study showed that when comparing pathological cerebral arteries to control groups, all control groups presented similar responses in the activation of immunological processes, the regulation of intracellular signaling pathways, and extracellular matrix productions, despite their intrinsic biological differences. When compared to STA, AU exhibited upregulation of stress and apoptosis genes, whereas MCA showed upregulation of genes associated with tRNA/rRNA processing. Moreover, our results suggest that the matched case-control study design, which involves control STA samples collected from the same subjects of matched aneurysm samples in our study, can improve the identification of non-inherited disease-associated genes. Given the challenges associated with obtaining fresh intracranial arteries from healthy individuals, our study suggests that using MCA, AU, or paired STA samples as controls are feasible strategies for future large-scale studies investigating cerebral vasculopathies. However, the intrinsic differences of each type of control should be taken into consideration when interpreting the results. With the limitations of each control type, it may be most optimal to use multiple tissues as controls.

Identifiants

DOI: 10.1007/s12975-023-01186-w PMID: 37612482

pubmed: 37612482

doi: 10.1007/s12975-023-01186-w

pii: 10.1007/s12975-023-01186-w

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Subventions

Organisme : NIH HHS

ID : 1R01 NS105675

Pays : United States

Informations de copyright

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