A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis.
high-throughput screening
human organoid culture
microarray 3D bioprinting
microphysiological system
perfusion plate
pillar plate
Journal
Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613
Informations de publication
Date de publication:
24 Aug 2023
24 Aug 2023
Historique:
revised:
10
08
2023
received:
02
08
2023
pubmed:
24
8
2023
medline:
24
8
2023
entrez:
24
8
2023
Statut:
aheadofprint
Résumé
Human organoids have the potential to revolutionize in vitro disease modeling by providing multicellular architecture and function that are similar to those in vivo. This innovative and evolving technology, however, still suffers from assay throughput and reproducibility to enable high-throughput screening (HTS) of compounds due to cumbersome organoid differentiation processes and difficulty in scale-up and quality control. Using organoids for HTS is further challenged by the lack of easy-to-use fluidic systems that are compatible with relatively large organoids. Here, these challenges are overcome by engineering "microarray three-dimensional (3D) bioprinting" technology and associated pillar and perfusion plates for human organoid culture and analysis. High-precision, high-throughput stem cell printing, and encapsulation techniques are demonstrated on a pillar plate, which is coupled with a complementary deep well plate and a perfusion well plate for static and dynamic organoid culture. Bioprinted cells and spheroids in hydrogels are differentiated into liver and intestine organoids for in situ functional assays. The pillar/perfusion plates are compatible with standard 384-well plates and HTS equipment, and thus may be easily adopted in current drug discovery efforts.
Identifiants
pubmed: 37616035
doi: 10.1002/adhm.202302502
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2302502Subventions
Organisme : NIDDK NIH HHS
ID : UG3 DK119982
Pays : United States
Organisme : NCATS NIH HHS
ID : R44 TR003491
Pays : United States
Organisme : NIDDK NIH HHS
ID : UH3 DK119982
Pays : United States
Organisme : NCATS NIH HHS
ID : R44TR003491
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01 ES025779
Pays : United States
Organisme : NIDDK NIH HHS
ID : UH3DK119982
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01ES025779
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.
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