Fluorinated Silane-Modified Filtroporation Devices Enable Gene Knockout in Human Hematopoietic Stem and Progenitor Cells.

Humans Silanes Gene Knockout Techniques Stem Cells Cell Culture Techniques Cell- and Tissue-Based Therapy
filtroporation gene knockout gene therapy hematopoietic stem cells intracellular delivery

Journal

ACS applied materials & interfaces
ISSN: 1944-8252
Titre abrégé: ACS Appl Mater Interfaces
Pays: United States
ID NLM: 101504991

Informations de publication

Date de publication:
06 Sep 2023
Historique:
medline: 7 9 2023
pubmed: 24 8 2023
entrez: 24 8 2023
Statut: ppublish

Résumé

Intracellular delivery technologies that are cost-effective, non-cytotoxic, efficient, and cargo-agnostic are needed to enable the manufacturing of cell-based therapies as well as gene manipulation for research applications. Current technologies capable of delivering large cargoes, such as plasmids and CRISPR-Cas9 ribonucleoproteins (RNPs), are plagued with high costs and/or cytotoxicity and often require substantial specialized equipment and reagents, which may not be available in resource-limited settings. Here, we report an intracellular delivery technology that can be assembled from materials available in most research laboratories, thus democratizing access to intracellular delivery for researchers and clinicians in low-resource areas of the world. These filtroporation devices permeabilize cells by pulling them through the pores of a cell culture insert by the application of vacuum available in biosafety cabinets. In a format that costs less than $10 in materials per experiment, we demonstrate the delivery of fluorescently labeled dextran, expression plasmids, and RNPs for gene knockout to Jurkat cells and human CD34

Identifiants

DOI: 10.1021/acsami.3c07045 PMID: 37616579 PMC: PMC10485797
pubmed: 37616579
doi: 10.1021/acsami.3c07045
pmc: PMC10485797
doi:

Substances chimiques

Silanes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

41299-41309

Subventions

Organisme : NIH HHS
ID : DP5 OD028181
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008042
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM067555
Pays : United States

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Auteurs

Isaura M Frost (IM)

Department of Bioengineering, University of California, Los Angeles, Los Angeles, California 90095, United States.
UCLA Medical Scientist Training Program, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095, United States.
Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095, United States.
ORCID: 0000-0003-2851-1008

Alexandra M Mendoza (AM)

Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.

Tzu-Ting Chiou (TT)

Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095, United States.

Philseok Kim (P)

John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, United States.
ORCID: 0000-0001-5814-2675

Joanna Aizenberg (J)

John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, United States.
ORCID: 0000-0002-2343-8705

Donald B Kohn (DB)

Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California 90095, United States.
Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, California 90095, United States.
Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, California 90095, United States.

Satiro N De Oliveira (SN)

Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095, United States.
ORCID: 0000-0002-8181-7316

Paul S Weiss (PS)

Department of Bioengineering, University of California, Los Angeles, Los Angeles, California 90095, United States.
Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States.
ORCID: 0000-0001-5527-6248

Steven J Jonas (SJ)

Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095, United States.
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, California 90095, United States.
Children's Discovery and Innovation Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
ORCID: 0000-0002-8111-0249

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Fluorinated Silane-Modified Filtroporation...
questionsmedicales.fr › Produits chimiques inorganiques › Composés du silicium › Silanes › Fluorinated Silane-Modified Filtroporation...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Techniques de knock-out de gènes › Fluorinated Silane-Modified Filtroporation...
questionsmedicales.fr › Cellules › Cellules souches › Fluorinated Silane-Modified Filtroporation...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Fluorinated Silane-Modified Filtroporation...
questionsmedicales.fr › Thérapeutique › Biothérapie › Thérapie cellulaire et tissulaire › Fluorinated Silane-Modified Filtroporation...