Determination of binding constants by ultrafast affinity extraction: Theoretical and experimental studies of optimum conditions for analysis.
Affinity microcolumn
Association equilibrium constant
Drug-protein binding
Free drug fraction
Ultrafast affinity extraction
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
27 Sep 2023
27 Sep 2023
Historique:
received:
18
05
2023
revised:
14
08
2023
accepted:
16
08
2023
medline:
7
9
2023
pubmed:
24
8
2023
entrez:
24
8
2023
Statut:
ppublish
Résumé
Ultrafast affinity extraction (UAE) is a form of microscale affinity HPLC that can be employed to quickly measure equilibrium constants for solute-binding agent interactions in solution. This study used chromatographic and equilibrium theory with universal plots to examine the general conditions that are needed in UAE to obtain accurate, precise, and robust measurements of equilibrium constants for such interactions. The predicted results were compared to those obtained by UAE in studies that examined the binding of various drugs with two transport proteins: human serum albumin and α
Identifiants
pubmed: 37619255
pii: S0021-9673(23)00532-0
doi: 10.1016/j.chroma.2023.464307
pii:
doi:
Substances chimiques
Gliclazide
G4PX8C4HKV
Orosomucoid
0
Serum Albumin, Human
ZIF514RVZR
Warfarin
5Q7ZVV76EI
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
464307Informations de copyright
Copyright © 2023. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: David Hage reports financial support was provided by National Science Foundation. David Hage has patent #Analysis of Free Analyte Fractions by Rapid Affinity Chromatography, U.S. Patent 6720,193 issued to David S. Hage, William A. Clarke.