Does microplastic analysis method affect our understanding of microplastics in the environment?

Two analytical methods - both in active use at different laboratories - were tested and compared against each other to investigate how the procedure influences microplastic (MP) detection with micro Fourier Transform Infrared Spectroscopy (μFTIR) imaging. A representative composite water sample collected from the Danube River was divided into 12 subsamples, and processed following two different methods, which differed in MP isolation procedures, the optical substrate utilized for the chemical imaging, and the detection limit of the spectroscopic instruments. The first instrument had a nominal pixel resolution of 5.5 μm, while the second had a nominal resolution of 25 μm. These two methods led to different MP abundance, MP mass estimates, but not MP characteristics. Only looking at MPs > 50 μm, the first method showed a higher MP abundance, namely 418-2571 MP m

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Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.