Molecular architecture and conservation of an immature human endogenous retrovirus.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
24 08 2023
24 08 2023
Historique:
received:
14
01
2023
accepted:
09
08
2023
medline:
28
8
2023
pubmed:
25
8
2023
entrez:
24
8
2023
Statut:
epublish
Résumé
The human endogenous retrovirus K (HERV-K) is the most recently acquired endogenous retrovirus in the human genome and is activated and expressed in many cancers and amyotrophic lateral sclerosis. We present the immature HERV-K capsid structure at 3.2 Å resolution determined from native virus-like particles using cryo-electron tomography and subtomogram averaging. The structure shows a hexamer unit oligomerized through a 6-helix bundle, which is stabilized by a small molecule analogous to IP6 in immature HIV-1 capsid. The HERV-K immature lattice is assembled via highly conserved dimer and trimer interfaces, as detailed through all-atom molecular dynamics simulations and supported by mutational studies. A large conformational change mediated by the linker between the N-terminal and the C-terminal domains of CA occurs during HERV-K maturation. Comparison between HERV-K and other retroviral immature capsid structures reveals a highly conserved mechanism for the assembly and maturation of retroviruses across genera and evolutionary time.
Identifiants
pubmed: 37620323
doi: 10.1038/s41467-023-40786-w
pii: 10.1038/s41467-023-40786-w
pmc: PMC10449913
doi:
Substances chimiques
Capsid Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
5149Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023. Springer Nature Limited.
Références
Nature. 2018 Aug;560(7719):509-512
pubmed: 30069050
Nat Commun. 2021 Mar 30;12(1):1957
pubmed: 33785757
J Comput Chem. 2010 Jan 30;31(2):455-61
pubmed: 19499576
Nat Commun. 2019 Dec 20;10(1):5822
pubmed: 31862888
PLoS Pathog. 2007 Jan;3(1):e10
pubmed: 17257061
J Chem Theory Comput. 2017 Jun 13;13(6):3031-3048
pubmed: 28430426
Am J Pathol. 1996 Nov;149(5):1727-35
pubmed: 8909261
Genome Res. 2006 Dec;16(12):1548-56
pubmed: 17077319
J Virol. 2010 Nov;84(21):11555-9
pubmed: 20739518
Sci Transl Med. 2015 Sep 30;7(307):307ra153
pubmed: 26424568
J Virol. 2016 Aug 26;90(18):8074-84
pubmed: 27356903
J Virol. 2010 Jul;84(13):6276-87
pubmed: 20392845
Methods. 2009 Oct;49(2):174-80
pubmed: 19398010
J Virol. 2015 Oct;89(20):10294-302
pubmed: 26223638
Nat Commun. 2021 May 28;12(1):3226
pubmed: 34050170
Commun Biol. 2021 Apr 16;4(1):481
pubmed: 33863979
IEEE Trans Image Process. 2005 Sep;14(9):1314-23
pubmed: 16190467
Structure. 2012 Dec 5;20(12):2003-13
pubmed: 23217682
J Virol. 2006 Nov;80(21):10752-62
pubmed: 17041225
Immun Ageing. 2020 May 13;17:11
pubmed: 32435269
J Mol Graph. 1996 Feb;14(1):33-8, 27-8
pubmed: 8744570
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Viruses. 2021 Dec 29;14(1):
pubmed: 35062258
J Struct Biol. 2017 Feb;197(2):102-113
pubmed: 27444392
J Virol. 2017 Dec 14;92(1):
pubmed: 29046457
Nucleic Acids Res. 2022 Jul 5;50(W1):W276-W279
pubmed: 35412617
PLoS Pathog. 2020 Jan 27;16(1):e1008277
pubmed: 31986188
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
pubmed: 20057044
Mol Biol Evol. 2009 Nov;26(11):2617-26
pubmed: 19666991
Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11751-E11760
pubmed: 30478053
Proteins. 2003 Feb 15;50(3):437-50
pubmed: 12557186
Cell. 2023 Jan 19;186(2):287-304.e26
pubmed: 36610399
Int J Cancer. 2010 Jan 15;126(2):306-14
pubmed: 19795446
Front Oncol. 2021 May 13;11:658489
pubmed: 34055625
Cancer Res. 2005 May 15;65(10):4172-80
pubmed: 15899808
Nat Methods. 2020 Mar;17(3):328-334
pubmed: 32042190
J Comput Chem. 2005 Dec;26(16):1781-802
pubmed: 16222654
J Virol. 2018 Dec 10;93(1):
pubmed: 30305351
Virus Evol. 2020 Feb 12;6(1):veaa003
pubmed: 32064119
Crit Rev Microbiol. 2018 Nov;44(6):715-738
pubmed: 30318978
J Chem Inf Model. 2012 Dec 21;52(12):3144-54
pubmed: 23146088
Nature. 2015 Jan 22;517(7535):505-8
pubmed: 25363765
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5503-7
pubmed: 6291047
J Virol. 1996 Aug;70(8):5695-700
pubmed: 8764091
J Mol Biol. 1979 Oct 15;134(1):109-42
pubmed: 537059
J Virol. 1999 Oct;73(10):8073-82
pubmed: 10482556
Nature. 2001 Feb 15;409(6822):860-921
pubmed: 11237011
Structure. 2008 May;16(5):673-83
pubmed: 18462672
Retrovirology. 2011 May 09;8:30
pubmed: 21554716
Nat Methods. 2019 Nov;16(11):1161-1168
pubmed: 31611690
Viruses. 2016 Apr 18;8(4):103
pubmed: 27096867
Viruses. 2021 Jan 18;13(1):
pubmed: 33477490
Protein Sci. 2022 Dec;31(12):e4472
pubmed: 36251681
Nat Methods. 2017 Jan;14(1):71-73
pubmed: 27819658
Structure. 2013 Oct 8;21(10):1735-42
pubmed: 24035711
J Chem Inf Model. 2012 Dec 21;52(12):3155-68
pubmed: 23145473
J Virol. 2003 Sep;77(17):9474-85
pubmed: 12915562
J Virol. 2014 Dec;88(24):14148-60
pubmed: 25275119
Retrovirology. 2011 Mar 23;8:21
pubmed: 21429186
Nat Commun. 2016 Dec 13;7:13689
pubmed: 27958264
J Virol. 1993 Nov;67(11):6778-87
pubmed: 7692084