Cross-species comparative analysis of single presynapses.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 08 2023
24 08 2023
Historique:
received:
13
03
2023
accepted:
16
08
2023
medline:
28
8
2023
pubmed:
25
8
2023
entrez:
24
8
2023
Statut:
epublish
Résumé
Comparing brain structure across species and regions enables key functional insights. Leveraging publicly available data from a novel mass cytometry-based method, synaptometry by time of flight (SynTOF), we applied an unsupervised machine learning approach to conduct a comparative study of presynapse molecular abundance across three species and three brain regions. We used neural networks and their attractive properties to model complex relationships among high dimensional data to develop a unified, unsupervised framework for comparing the profile of more than 4.5 million single presynapses among normal human, macaque, and mouse samples. An extensive validation showed the feasibility of performing cross-species comparison using SynTOF profiling. Integrative analysis of the abundance of 20 presynaptic proteins revealed near-complete separation between primates and mice involving synaptic pruning, cellular energy, lipid metabolism, and neurotransmission. In addition, our analysis revealed a strong overlap between the presynaptic composition of human and macaque in the cerebral cortex and neostriatum. Our unique approach illuminates species- and region-specific variation in presynapse molecular composition.
Identifiants
pubmed: 37620363
doi: 10.1038/s41598-023-40683-8
pii: 10.1038/s41598-023-40683-8
pmc: PMC10449792
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
13849Subventions
Organisme : NIA NIH HHS
ID : UF1 AG057707
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG077443
Pays : United States
Organisme : NIA NIH HHS
ID : P30 AG066509
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM138353
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR027431
Pays : United States
Organisme : NIA NIH HHS
ID : P30 AG072947
Pays : United States
Organisme : NIA NIH HHS
ID : U19 AG066567
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL087103
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG056287
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG057915
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL122393
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG068279
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG058829
Pays : United States
Organisme : NIA NIH HHS
ID : P30 AG049638
Pays : United States
Informations de copyright
© 2023. Springer Nature Limited.
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