Multiphoton intravital microscopy of rodents.
Journal
Nature reviews. Methods primers
ISSN: 2662-8449
Titre abrégé: Nat Rev Methods Primers
Pays: England
ID NLM: 101778010
Informations de publication
Date de publication:
2022
2022
Historique:
medline:
1
1
2022
pubmed:
1
1
2022
entrez:
25
8
2023
Statut:
ppublish
Résumé
Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular and subcellular resolution. IVM experiments have revealed unique insights into the dynamic interplay between different cell types and their local environment, and how this drives morphogenesis and homeostasis of tissues, inflammation and immune responses, and the development of various diseases. This Primer introduces researchers to IVM technologies, with a focus on multiphoton microscopy of rodents, and discusses challenges, solutions and practical tips on how to perform IVM. To illustrate the unique potential of IVM, several examples of results are highlighted. Finally, we discuss data reproducibility and how to handle big imaging data sets.
Identifiants
pubmed: 37621948
doi: 10.1038/s43586-022-00168-w
pmc: PMC10449057
mid: NIHMS1867259
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NCI NIH HHS
ID : R01 CA216248
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA255153
Pays : United States
Déclaration de conflit d'intérêts
Competing interests P.T. receives reagents from Kadmon, InxMed (also consultant), Redx Pharma, Équilibre Biopharmaceuticals and Amplia Therapeutics. Under a licensing agreement between Amplia Therapeutics and Garvan Institute of Medical Research, D.H. and P.T. (consultant) are entitled to milestone payments. All other authors declare no competing interests.
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