Does dinocyst wall composition really reflect trophic affinity? New evidence from ATR micro-FTIR spectroscopy measurements.
attenuated total reflection micro-Fourier transform infrared spectroscopy
bleaching
dinosporin composition
optical photothermal infrared spectroscopy
organic-walled dinocysts
pigments
spectrochemical methods
sunscreen
synchrotron radiation
trophic affinity
Journal
Journal of phycology
ISSN: 1529-8817
Titre abrégé: J Phycol
Pays: United States
ID NLM: 9882935
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
revised:
02
05
2023
received:
22
12
2022
accepted:
21
07
2023
pubmed:
25
8
2023
medline:
25
8
2023
entrez:
25
8
2023
Statut:
ppublish
Résumé
Attenuated total reflection (ATR) microscope Fourier transform infrared (micro-FTIR) spectroscopy was used to investigate the dinosporin composition in the walls of modern, organic-walled dinoflagellate resting cysts (dinocysts). Variable cyst wall compositions were observed, which led to the erection of four spectrochemical groups, some with striking similarities to other resistant biomacromolecules such as sporopollenin and algaenan. Furthermore, possible proxies derivable from the spectrochemical composition of modern and fossil dinocysts were discussed. The color of the dinocyst walls was reflected in the spectral data. When comparing that color with a standard and the results of a series of bleaching experiments with oxidative agents, eumelanin was assigned as a likely pigment contributing to the observed color. Following this assignment, the role of eumelanin as an ultraviolet sunscreen in colored dinocysts was hypothesized, and its implications on the autofluorescence and morphological preservation of dinocysts were further discussed. Unlike what had previously been assumed, it was shown that micro-FTIR data from dinocysts cannot be used to unambiguously infer trophic affinities of their associated cells. Finally, using methods with high spatial resolutions (synchrotron transmission micro-FTIR and optical photothermal infrared spectroscopy), it was shown that dinocyst wall layers are chemically homogenous at the probed scales. This study fills a large knowledge gap in our understanding of the chemical nature of dinocyst walls and has nuanced certain assumptions and interpretations made in the past.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1064-1084Subventions
Organisme : Fonds Wetenschappelijk Onderzoek
ID : K210522N
Organisme : Herculesstichting
ID : FT-IMAGER Project-AUGE/13/16
Informations de copyright
© 2023 Phycological Society of America.
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