Blood Clotting Dissolution in the Presence of a Magnetic Field and Preliminary Study with MG63 Osteoblast-like Cells-Further Developments for Guided Bone Regeneration?
MG63 osteoblast-like cells
clot stability
fibrinolysis
guided bone regeneration
static magnetic field
tissue-type plasminogen activator
trypsin
Journal
Bioengineering (Basel, Switzerland)
ISSN: 2306-5354
Titre abrégé: Bioengineering (Basel)
Pays: Switzerland
ID NLM: 101676056
Informations de publication
Date de publication:
26 Jul 2023
26 Jul 2023
Historique:
received:
26
05
2023
revised:
21
07
2023
accepted:
24
07
2023
medline:
26
8
2023
pubmed:
26
8
2023
entrez:
26
8
2023
Statut:
epublish
Résumé
The influence of a magnetic field on the activation of bone cells and remodelling of alveolar bone is known to incite bone regeneration. Guided Bone Regeneration (GBR) aims to develop biomimetic scaffolds to allow for the functioning of the barrier and the precise succession of wound healing steps, including haemostasis. The effect of a magnetic field on blood clot dissolution has not been studied yet. We conducted a methodological study on the clot stability in the presence of a static magnetic field (SMF). Preformed whole blood (WB) clots were treated with either a broad proteolytic enzyme (trypsin) or a specific fibrinolytic agent, i.e., tissue-type plasminogen activator (t-PA). MG63 osteoblast-like cells were added to preformed WB clots to assess cell proliferation. After having experienced a number of clotting and dissolution protocols, we obtained clot stability exerted by SMF when tissue factor (for clotting) and t-PA + plasminogen (for fibrinolysis) were used. WB clots allowed osteoblast-like cells to survive and proliferate, however no obvious effects of the magnetic field were noted. Paramagnetic properties of erythrocytes may have influenced the reduction in clot dissolution. Future studies are warranted to fully exploit the combination of magnetic forces, WB clot and cells in GBR applied to orthodontics and prosthodontics.
Sections du résumé
BACKGROUND
BACKGROUND
The influence of a magnetic field on the activation of bone cells and remodelling of alveolar bone is known to incite bone regeneration. Guided Bone Regeneration (GBR) aims to develop biomimetic scaffolds to allow for the functioning of the barrier and the precise succession of wound healing steps, including haemostasis. The effect of a magnetic field on blood clot dissolution has not been studied yet.
METHODS
METHODS
We conducted a methodological study on the clot stability in the presence of a static magnetic field (SMF). Preformed whole blood (WB) clots were treated with either a broad proteolytic enzyme (trypsin) or a specific fibrinolytic agent, i.e., tissue-type plasminogen activator (t-PA). MG63 osteoblast-like cells were added to preformed WB clots to assess cell proliferation.
RESULTS
RESULTS
After having experienced a number of clotting and dissolution protocols, we obtained clot stability exerted by SMF when tissue factor (for clotting) and t-PA + plasminogen (for fibrinolysis) were used. WB clots allowed osteoblast-like cells to survive and proliferate, however no obvious effects of the magnetic field were noted.
CONCLUSIONS
CONCLUSIONS
Paramagnetic properties of erythrocytes may have influenced the reduction in clot dissolution. Future studies are warranted to fully exploit the combination of magnetic forces, WB clot and cells in GBR applied to orthodontics and prosthodontics.
Identifiants
pubmed: 37627773
pii: bioengineering10080888
doi: 10.3390/bioengineering10080888
pmc: PMC10451701
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Osteophenix Italia
ID : N/A
Références
Front Chem. 2020 Sep 25;8:745
pubmed: 33102429
Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Jul;84(1 Pt 1):011905
pubmed: 21867211
Nanomedicine. 2019 Oct;21:102046
pubmed: 31279063
Clin Implant Dent Relat Res. 2003;5(2):78-81
pubmed: 14536041
Evid Based Complement Alternat Med. 2011;2011:
pubmed: 20953437
Bioelectromagnetics. 2015 Apr;36(4):267-76
pubmed: 25808160
TH Open. 2018 Jun 07;2(2):e218-e228
pubmed: 31249945
Sci Rep. 2016 Oct 21;6:35645
pubmed: 27767056
J Biomed Mater Res A. 2018 Dec;106(12):3042-3052
pubmed: 30194699
J Oral Maxillofac Res. 2019 Sep 05;10(3):e2
pubmed: 31620264
Tissue Eng Part C Methods. 2013 Sep;19(9):720-9
pubmed: 23327780
Tissue Eng Part B Rev. 2010 Aug;16(4):427-34
pubmed: 20196645
Sci Rep. 2017 May 24;7(1):2346
pubmed: 28539608
Biotechnol Bioeng. 2021 Nov;118(11):4217-4230
pubmed: 34264518
Clin Orthop Relat Res. 2010 Aug;468(8):2260-77
pubmed: 20387020
J Periodontal Res. 2005 Jun;40(3):269-76
pubmed: 15853974
J Biol Regul Homeost Agents. 2013 Apr-Jun;27(2):531-41
pubmed: 23830402
J Biomater Sci Polym Ed. 2013;24(13):1529-48
pubmed: 23848447
Sci Rep. 2016 Jun 20;6:28119
pubmed: 27321930
J Periodontal Implant Sci. 2017 Oct;47(5):273-291
pubmed: 29093986
Langenbecks Arch Surg. 2013 Jan;398(1):161-7
pubmed: 23070478
Nature. 1965 Apr 24;206(982):420-2
pubmed: 5835725
Clin Oral Implants Res. 2003 Aug;14(4):455-63
pubmed: 12869008
Biomimetics (Basel). 2023 Mar 06;8(1):
pubmed: 36975336
Clin Oral Implants Res. 2010 Jun;21(6):567-76
pubmed: 20666785
Biomaterials. 2007 Feb;28(6):966-74
pubmed: 17095084
Am J Orthod Dentofacial Orthop. 1995 Jun;107(6):578-88
pubmed: 7771362
Int J Mol Sci. 2019 Dec 11;20(24):
pubmed: 31835696
Clin Oral Implants Res. 2003 Feb;14(1):63-71
pubmed: 12562367
Adv Drug Deliv Rev. 2015 Apr;84:1-29
pubmed: 25236302
Electromagn Biol Med. 2008;27(1):55-64
pubmed: 18327714
Clin Oral Implants Res. 2011 May;22(5):538-45
pubmed: 21198906
Am J Surg. 1957 Mar;93(3):385-7
pubmed: 13403056
J Tissue Eng Regen Med. 2017 Oct;11(10):2864-2875
pubmed: 27401283
J Cell Physiol. 2012 Feb;227(2):857-66
pubmed: 21830215
Eur J Oral Sci. 2017 Oct;125(5):315-337
pubmed: 28833567
Adv Healthc Mater. 2018 Nov;7(22):e1800700
pubmed: 30240157
Cells. 2021 Dec 28;11(1):
pubmed: 35011642
Biomaterials. 2011 Nov;32(31):7831-8
pubmed: 21820169
Physiol Chem Phys Med NMR. 1983;15(6):459-68
pubmed: 6676735
Materials (Basel). 2021 Nov 04;14(21):
pubmed: 34772167
Ultrasonics. 2019 Sep;98:62-71
pubmed: 31202970
Am J Orthod Dentofacial Orthop. 1987 Feb;91(2):91-104
pubmed: 3468800
Clin Oral Implants Res. 2013 Mar;24(3):312-9
pubmed: 22092958
Blood. 1993 Aug 15;82(4):1328-34
pubmed: 8353291
Thromb Haemost. 2009 Dec;102(6):1169-75
pubmed: 19967148
J Prosthodont Res. 2013 Jan;57(1):3-14
pubmed: 23347794
J Dent Res. 2003 Dec;82(12):962-6
pubmed: 14630895
J Med Case Rep. 2014 Dec 17;8:437
pubmed: 25515949
Tissue Eng Part B Rev. 2014 Dec;20(6):697-712
pubmed: 24906469
Bioelectromagnetics. 1996;17(4):335-8
pubmed: 8891193