Label-Free DNA Detection Using Etched Tilted Bragg Fiber Grating-Based Biosensor.

Reproducibility of Results DNA DNA, Complementary Fiber Optic Technology
DNA biosensor etching optical fiber sensor tilted fiber Bragg grating

Journal

Sensors (Basel, Switzerland)
ISSN: 1424-8220
Titre abrégé: Sensors (Basel)
Pays: Switzerland
ID NLM: 101204366

Informations de publication

Date de publication:
08 Aug 2023
Historique:
received: 19 06 2023
revised: 02 08 2023
accepted: 03 08 2023
medline: 28 8 2023
pubmed: 26 8 2023
entrez: 26 8 2023
Statut: epublish

Résumé

A label-free-based fiber optic biosensor based on etched tilted Bragg fiber grating (TFBG) is proposed and practically demonstrated. Conventional phase mask technic has been utilized to inscribe tilted fiber Bragg grating with a tilt angle of 10°, while the etching has been accomplished with hydrofluoric acid. A composite of polyethylenimine (PEI)/poly(acrylic acid) (PAA) has been thermally deposited on the etched TFBG, followed by immobilization of probe DNA (pDNA) on this deposited layer. The hybridization of pDNA with the complementary DNA (cDNA) has been monitored using wavelength-dependent interrogation. The reproducibility of the probes has been demonstrated by fabricating three identical probes and their response has been investigated for cDNA concentration ranging from 0 μM to 3 μM. The maximum sensitivity has been found to be 320 pm/μM, with the detection limit being 0.65 μM. Furthermore, the response of the probes towards non-cDNA has also been investigated in order to establish its specificity.

Identifiants

DOI: 10.3390/s23167019 PMID: 37631556 PMC: PMC10457823
pubmed: 37631556
pii: s23167019
doi: 10.3390/s23167019
pmc: PMC10457823
pii:
doi:

Substances chimiques

DNA 9007-49-2
DNA, Complementary 0
poly(ethyleneimine)-poly(acrylic acid) 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Shenzhen-HK-Macao Science and Technology Plan C
ID : SGDX2020110309520303

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Auteurs

Abdullah Al Noman (AA)

Department of Electronic and Information Engineering, Photonics Research Institute, The Hong Kong Polytechnic University, 11 Yuk Choi Rd, Hung Hom, Hong Kong SAR, China.
ORCID: 0000-0001-9984-8498

Jitendra Narayan Dash (JN)

Department of Electrical Engineering, Photonics Research Institute, The Hong Kong Polytechnic University, 11 Yuk Choi Rd, Hung Hom, Hong Kong SAR, China.

Md Abdullah Al Maruf (MAA)

Department of Health Technology and Informatics, The Hong Kong Polytechnic University, 11 Yuk Choi Rd, Hung Hom, Hong Kong SAR, China.

Cheng Xin (C)

Department of Electrical Engineering, Photonics Research Institute, The Hong Kong Polytechnic University, 11 Yuk Choi Rd, Hung Hom, Hong Kong SAR, China.
ORCID: 0000-0003-3678-3038

Hwa-Yam Tam (HY)

Department of Electrical Engineering, Photonics Research Institute, The Hong Kong Polytechnic University, 11 Yuk Choi Rd, Hung Hom, Hong Kong SAR, China.

Changyuan Yu (C)

Department of Electronic and Information Engineering, Photonics Research Institute, The Hong Kong Polytechnic University, 11 Yuk Choi Rd, Hung Hom, Hong Kong SAR, China.
ORCID: 0000-0002-3185-0441

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Label-Free DNA Detection Using Etched Tilted...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Label-Free DNA Detection Using Etched Tilted...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Sondes moléculaires › Sondes d'acide nucléique › Sondes d'ADN › ADN complémentaire › Label-Free DNA Detection Using Etched Tilted...
questionsmedicales.fr › Disciplines des sciences naturelles › Physique › Technologie des fibres optiques › Label-Free DNA Detection Using Etched Tilted...