Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets.
Cell Biology
Cell separation/fractionation
Gene Expression
Molecular Biology
Protein expression and purification
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
25 Aug 2023
25 Aug 2023
Historique:
received:
23
03
2023
revised:
12
06
2023
accepted:
31
07
2023
medline:
27
8
2023
pubmed:
27
8
2023
entrez:
26
8
2023
Statut:
aheadofprint
Résumé
Here, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated fraction. We then detail an RNA immunoprecipitation procedure adapted to identify chromatin-associated RNA targets. This protocol can be adapted to other human or mammalian chromatin-associated RNA binding proteins. For complete details on the use and execution of this protocol, please refer to Timcheva et al.
Identifiants
pubmed: 37632746
pii: S2666-1667(23)00495-1
doi: 10.1016/j.xpro.2023.102528
pmc: PMC10477737
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102528Informations de copyright
Copyright © 2023. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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