Bioorthogonal Caging-Group-Free Photoactivatable Probes for Minimal-Linkage-Error Nanoscopy.
Journal
ACS central science
ISSN: 2374-7943
Titre abrégé: ACS Cent Sci
Pays: United States
ID NLM: 101660035
Informations de publication
Date de publication:
23 Aug 2023
23 Aug 2023
Historique:
received:
20
06
2023
medline:
28
8
2023
pubmed:
28
8
2023
entrez:
28
8
2023
Statut:
epublish
Résumé
Here we describe highly compact, click compatible, and photoactivatable dyes for super-resolution fluorescence microscopy (nanoscopy). By combining the photoactivatable xanthone (PaX) core with a tetrazine group, we achieve minimally sized and highly sensitive molecular dyads for the selective labeling of unnatural amino acids introduced by genetic code expansion. We exploit the excited state quenching properties of the tetrazine group to attenuate the photoactivation rates of the PaX, and further reduce the overall fluorescence emission of the photogenerated fluorophore, providing two mechanisms of selectivity to reduce the off-target signal. Coupled with MINFLUX nanoscopy, we employ our dyads in the minimal-linkage-error imaging of vimentin filaments, demonstrating molecular-scale precision in fluorophore positioning.
Identifiants
pubmed: 37637742
doi: 10.1021/acscentsci.3c00746
pmc: PMC10450876
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1581-1590Informations de copyright
© 2023 The Authors. Published by American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare the following competing financial interest(s): R.L., M.L.B., and A.N.B. are co-inventors of a patent application covering the photoactivatable dyes of this work, filed by the Max Planck Society. S.W.H. owns shares of Abberior GmbH and Abberior Instruments GmbH whose dyes and MINFLUX microscope, respectively, have also been used in this study.
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