Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue.

Animals Mice Flow Cytometry Adipose Tissue, White Homeostasis Leukocytes Macrophages
Flow cytometry Macrophages White adipose tissue

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2024
Historique:
medline: 29 8 2023
pubmed: 28 8 2023
entrez: 28 8 2023
Statut: ppublish

Résumé

Macrophages are one of the prominent leukocyte populations in white adipose tissue (WAT) and play an important role during WAT homeostasis and remodeling. Macrophage function in WAT is determined by ontogeny and the local tissue environment. Here, we present a protocol to analyze different macrophage populations from murine WAT using flow cytometry.

Identifiants

DOI: 10.1007/978-1-0716-3437-0_10 PMID: 37639121
pubmed: 37639121
doi: 10.1007/978-1-0716-3437-0_10
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

149-158

Informations de copyright

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Rosen ED, Spiegelman BM (2014) What we talk about when we talk about fat. Cell 156:20–44
doi: 10.1016/j.cell.2013.12.012 pubmed: 24439368 pmcid: 3934003
Choe SS, Huh JY, Hwang IJ, Kim JI, Kim JB (2016) Adipose tissue remodeling: its role in energy metabolism and metabolic disorders. Front Endocrinol (Lausanne) 7:30
doi: 10.3389/fendo.2016.00030 pubmed: 27148161
Chakarov S, Bleriot C, Ginhoux F (2022) Role of adipose tissue macrophages in obesity-related disorders. J Exp Med 219:e20211948
doi: 10.1084/jem.20211948 pubmed: 35543703 pmcid: 9098652
Weisberg SP, McCann D, Desai M, Rosenbaum M, Leibel RL, Ferrante AW Jr (2003) Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest 112:1796–1808
doi: 10.1172/JCI200319246 pubmed: 14679176 pmcid: 296995
Cox N, Geissmann F (2020) Macrophage ontogeny in the control of adipose tissue biology. Curr Opin Immunol 62:1–8
doi: 10.1016/j.coi.2019.08.002 pubmed: 31670115
Cox N, Crozet L, Holtman IR, Loyher PL, Lazarov T, White JB, Mass E, Stanley ER, Elemento O, Glass CK et al (2021) Diet-regulated production of PDGFcc by macrophages controls energy storage. Science 373:eabe9383
doi: 10.1126/science.abe9383 pubmed: 34210853 pmcid: 9558257

Auteurs

Dalila Juliana Silva Ribeiro (DJ)

Institute of Innate Immunity, Department of Biophysical Imaging, Medical Faculty, University of Bonn, Bonn, Germany.

Seniz Yüksel (S)

Institute of Innate Immunity, Department of Biophysical Imaging, Medical Faculty, University of Bonn, Bonn, Germany.

Andreas Dolf (A)

Flow Cytometry Core Facility, Medical Faculty, University of Bonn, Bonn, Germany.

Dagmar Wachten (D)

Institute of Innate Immunity, Department of Biophysical Imaging, Medical Faculty, University of Bonn, Bonn, Germany. dwachten@uni-bonn.de.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Isolation and Flow Cytometry Analysis of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Isolation and Flow Cytometry Analysis of...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › Isolation and Flow Cytometry Analysis of...
questionsmedicales.fr › Tissus › Tissu conjonctif › Tissu adipeux › Tissu adipeux blanc › Isolation and Flow Cytometry Analysis of...
questionsmedicales.fr › Phénomènes physiologiques › Homéostasie › Isolation and Flow Cytometry Analysis of...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Isolation and Flow Cytometry Analysis of...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Isolation and Flow Cytometry Analysis of...