Automated Cell Counting of Macrophages In Situ.
Automatic cell count
Batch analysis
CellProfiler
Macrophages
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
29
8
2023
pubmed:
28
8
2023
entrez:
28
8
2023
Statut:
ppublish
Résumé
Using the open-source image analysis software CellProfiler to automatically quantify antibody-stained or fluorescently labeled macrophages in situ provides accurate and reproducible cell counts. It is a vastly enhanced alternative method to both manual cell counting and estimation of cell marker expression based on fluorescence intensity. Quantification of tissue-resident macrophages acquired on widefield or confocal microscopes can be batch processed using our pipeline to produce data within minutes.
Identifiants
pubmed: 37639144
doi: 10.1007/978-1-0716-3437-0_33
doi:
Substances chimiques
Antibodies
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
505-518Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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