Pneumococcal pneumonia and endotoxemia: An experimental and clinical reappraisal.
Streptococcus pneumoniae
endotoxemia
immune response
lipopolysaccharide
pneumonia
Journal
European journal of clinical investigation
ISSN: 1365-2362
Titre abrégé: Eur J Clin Invest
Pays: England
ID NLM: 0245331
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
revised:
20
07
2023
received:
12
06
2023
accepted:
02
08
2023
pubmed:
29
8
2023
medline:
29
8
2023
entrez:
29
8
2023
Statut:
ppublish
Résumé
Circulating endotoxins could result from bacterial digestive translocation during sepsis, thus contributing to uncontrolled systemic inflammation, leading in turn to organ dysfunction. We addressed this issue in the setting of severe pneumococcal pneumonia. Endotoxemia was measured in a clinically relevant rabbit model of ventilated pneumococcal pneumonia and in 110 patients with bacteraemic pneumonia, using a patented mass spectrometry (LC-MS/MS) method for detection of 3-OH fatty acids (C10, C12, C14, C16 and C18), which are molecules bound to the lipid A motif of LPS. Whereas higher levels of systemic inflammation and organ dysfunctions were found, there was no significant difference in lipopolysaccharide concentrations when infected rabbits were compared to non-infected ones, or when patients were compared to healthy volunteers. Seemingly, endotoxins do not drive the overwhelming inflammation associated with severe forms of pneumococcal pneumonia.
Sections du résumé
BACKGROUND
BACKGROUND
Circulating endotoxins could result from bacterial digestive translocation during sepsis, thus contributing to uncontrolled systemic inflammation, leading in turn to organ dysfunction. We addressed this issue in the setting of severe pneumococcal pneumonia.
METHODS
METHODS
Endotoxemia was measured in a clinically relevant rabbit model of ventilated pneumococcal pneumonia and in 110 patients with bacteraemic pneumonia, using a patented mass spectrometry (LC-MS/MS) method for detection of 3-OH fatty acids (C10, C12, C14, C16 and C18), which are molecules bound to the lipid A motif of LPS.
RESULTS
RESULTS
Whereas higher levels of systemic inflammation and organ dysfunctions were found, there was no significant difference in lipopolysaccharide concentrations when infected rabbits were compared to non-infected ones, or when patients were compared to healthy volunteers.
CONCLUSIONS
CONCLUSIONS
Seemingly, endotoxins do not drive the overwhelming inflammation associated with severe forms of pneumococcal pneumonia.
Types de publication
Letter
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14077Subventions
Organisme : (PHRC) 2004/37 (HyaloStrepto project)
Organisme : AOI (Appel d'Offre Interne) from the University hospital of Dijon
Organisme : INSERM (Institut National de la Sante et de la Recherche Medicale - Center de Recherche UMR 1231, Dijon, France), the national research agency (ANR) Investissements d'Avenir Grant (ANR-11 LABX-0021-01, Labex Lipstic, Dijon, France), and the Université Bourgogne Franche Comte (Dijon, France).
Organisme : MSD-Avenir 2018 (Paris, France; sponsorship agreement) (experimental project)
Investigateurs
Delphine Croisier
(D)
Sandrine Gohier
(S)
Carole Charles
(C)
Adrien Guilloteau
(A)
Marc Bardou
(M)
Ines Ben Guezala
(IB)
Jeanne Godon
(J)
Pierre-Emmanuel Charles
(PE)
Sylvie Nguyen
(S)
Thomas Gauthier
(T)
Jean-Paul Pais de Barros
(JP)
Hélène Choubley
(H)
Victoria Bergas
(V)
Marine Jacquier
(M)
Jennifer Tetu
(J)
Jean-Pierre Quenot
(JP)
Maxime Luu
(M)
Christine Binquet
(C)
David Masson
(D)
Lionel Piroth
(L)
Mathieu Blot
(M)
Informations de copyright
© 2023 Stichting European Society for Clinical Investigation Journal Foundation. Published by John Wiley & Sons Ltd.
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