Mechanism of substrate binding and transport in BASS transporters.

Membrane transporter bile acid transporter crystal structure elevator mechanism

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
16 Aug 2023
Historique:
pubmed: 30 8 2023
medline: 30 8 2023
entrez: 30 8 2023
Statut: epublish

Résumé

The Bile Acid Sodium Symporter (BASS) family transports a wide array of molecules across membranes, including bile acids in humans, and small metabolites in plants. These transporters, many of which are sodium-coupled, have been shown to use an elevator mechanism of transport, but exactly how substrate binding is coupled to sodium ion binding and transport is not clear. Here we solve the crystal structure at 2.3 Å of a transporter from

Identifiants

pubmed: 37645971
doi: 10.1101/2023.06.02.543391
pmc: PMC10461908
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM118772
Pays : United States

Commentaires et corrections

Type : UpdateIn

Déclaration de conflit d'intérêts

Competing interests: Authors declare no competing interests.

Auteurs

Patrick Becker (P)

School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, U.K.

Fiona B Naughton (FB)

Department of Physics, Arizona State University, Tempe, AZ 85287.

Deborah H Brotherton (DH)

School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, U.K.

Raul Pacheco-Gomez (R)

Malvern Panalytical Ltd, Malvern, UK.

Oliver Beckstein (O)

Department of Physics, Arizona State University, Tempe, AZ 85287.

Alexander D Cameron (AD)

School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, U.K.

Classifications MeSH