Salivary pellicle modulates biofilm formation on titanium surfaces.
Biofilm formation
Dental enamel
Dental implants
Oral bacteria
Salivary pellicle
Titanium surfaces
Journal
Clinical oral investigations
ISSN: 1436-3771
Titre abrégé: Clin Oral Investig
Pays: Germany
ID NLM: 9707115
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
14
05
2023
accepted:
18
08
2023
medline:
9
10
2023
pubmed:
30
8
2023
entrez:
30
8
2023
Statut:
ppublish
Résumé
The present study aimed to evaluate the potential of the salivary pellicle (SP) formed on titanium (Ti) surfaces to modulate the formation of a biofilm composed of Streptococcus gordonii, Actinomyces naeslundii, Fusobacterium nucleatum, and Porphyromonas gingivalis. Ti substrates were incubated for 2 h with a pool of saliva samples obtained from 10 systemically and periodontally healthy subjects. Enamel substrates were included as a biological reference. Scanning electron microscopy (SEM) and Raman spectroscopy analysis were used to analyze the formation of the salivary pellicle. After the SP formation, the surfaces were incubated for 12 h with a mix of Streptococcus gordonii, Actinomyces naeslundii, Fusobacterium nucleatum, and Porphyromonas gingivalis. The number of bacterial cells attached to each surface was determined by the XTT assay while bacterial viability was analyzed by fluorescence microscopy using the LIVE/DEAD® BacLight The SEM and Raman spectroscopy analysis confirmed the presence of a salivary pellicle formed on the tested surfaces. Regarding the biofilm formation, the presence of the SP decreases the number of the bacterial cells detected in the test surfaces, compared with the uncover substrates. Even more, the SP-covered substrates showed similar bacterial counts in both Ti and enamel surfaces, meaning that the physicochemical differences of the substrates were less determinant than the presence of the SP. While on the SP-uncover substrates, differences in the bacterial adhesion patterns were directly related to the physicochemical nature of the substrates. The salivary pellicle was the main modulator in the development of the biofilm consisting of representative oral bacteria on the Ti substrates. The results of this study provide valuable information on the modulatory effect of the salivary pellicle on biofilm formation; such information allows us to understand better the events involved in the formation of oral biofilms on Ti dental implants.
Identifiants
pubmed: 37646908
doi: 10.1007/s00784-023-05230-9
pii: 10.1007/s00784-023-05230-9
pmc: PMC10560156
doi:
Substances chimiques
Titanium
D1JT611TNE
Types de publication
Journal Article
Langues
eng
Pagination
6135-6145Informations de copyright
© 2023. The Author(s).
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