Dihydrochalcone glycoside biosynthesis in Malus is regulated by two MYB-like transcription factors and is required for seed development.
Malus
dihydrochalcone glycoside
genetic regulation
genotype
promoter
seed
tissue
transcription factor
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
16
08
2023
received:
31
05
2023
accepted:
18
08
2023
medline:
29
11
2023
pubmed:
31
8
2023
entrez:
30
8
2023
Statut:
ppublish
Résumé
Dihydrochalcones (DHCs) including phlorizin (phloretin 2'-O-glucoside) and its positional isomer trilobatin (phloretin 4'-O-glucoside) are the most abundant phenylpropanoids in apple (Malus spp.). Transcriptional regulation of DHC production is poorly understood despite their importance in insect- and pathogen-plant interactions in human physiology research and in pharmaceuticals. In this study, segregation in hybrid populations and bulked segregant analysis showed that the synthesis of phlorizin and trilobatin in Malus leaves are both single-gene-controlled traits. Promoter sequences of PGT1 and PGT2, two glycosyltransferase genes involved in DHC glycoside synthesis, were shown to discriminate Malus with different DHC glycoside patterns. Differential PGT1 and PGT2 promoter activities determined DHC glycoside accumulation patterns between genotypes. Two transcription factors containing MYB-like DNA-binding domains were then shown to control DHC glycoside patterns in different tissues, with PRR2L mainly expressed in leaf, fruit, flower, stem, and seed while MYB8L mainly expressed in stem and root. Further hybridizations between specific genotypes demonstrated an absolute requirement for DHC glycoside production in Malus during seed development which explains why no Malus spp. with a null DHC chemotype have been reported.
Substances chimiques
trilobatin
23298I791N
dihydrochalcone
H5W525SX7Q
Phlorhizin
CU9S17279X
Transcription Factors
0
Phloretin
S5J5OE47MK
Glucosides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1492-1507Subventions
Organisme : National Natural Science Foundation of China
ID : 31972366
Organisme : the National Key R&D Program of China
ID : 2018YFD1000200
Informations de copyright
© 2023 Society for Experimental Biology and John Wiley & Sons Ltd.
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