Comparative Morphological Analysis of MCF10A and MCF7 Cells Using Holographic Time-lapse Microscopy.
Breast cancer
digital holographic microscopy
holomonitor
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
15
06
2023
revised:
17
07
2023
accepted:
21
07
2023
medline:
1
9
2023
pubmed:
31
8
2023
entrez:
30
8
2023
Statut:
ppublish
Résumé
Breast cancer, one of the most prevalent cancers globally, is marked by its cellular heterogeneity. A key aspect of breast cancer research is understanding the distinct morphological features of cancerous and non-cancerous cells, which could serve as potential targets for novel therapeutic interventions. In this light, our study aimed to comprehensively analyze the morphological features of the MCF10A and MCF7 cell lines, representing normal breast and breast cancer cells, respectively. The ultimate objective was to identify the most significant features that differentiate these cell lines. We utilized advanced imaging techniques such as holographic time-lapse microscopy, which provides real-time, three-dimensional imaging of cells, to conduct our comparative analysis. This allowed us to examine dynamic cellular morphology and behavior with exceptional sensitivity and resolution over time. The primary features assessed in our study included texture clustershade, area (μm Our findings highlighted significant differences in the morphological features of MCF10A and MCF7 cells. MCF10A cells showed a higher texture clustershade value, suggesting less symmetry than MCF7 cells. On the other hand, MCF7 cells had smaller cellular area, higher eccentricity, lower irregularity, higher phase shift sum, higher optical volume, higher shape convexity, and higher hull convexity compared to MCF10A cells. These results suggest that MCF7 cells are smaller, more circular, less irregular, exhibit different light properties, and have a closer to perfect 3D shape relative to MCF10A cells. The identified morphological differences between MCF10A and MCF7 cells offer valuable insights into the characteristics distinguishing normal breast cells from breast cancer cells. These findings not only contribute to our understanding of the morphological variability in breast cancer but also underscore the potential utility of these differences in future cancer diagnostics and treatment strategies.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Breast cancer, one of the most prevalent cancers globally, is marked by its cellular heterogeneity. A key aspect of breast cancer research is understanding the distinct morphological features of cancerous and non-cancerous cells, which could serve as potential targets for novel therapeutic interventions. In this light, our study aimed to comprehensively analyze the morphological features of the MCF10A and MCF7 cell lines, representing normal breast and breast cancer cells, respectively. The ultimate objective was to identify the most significant features that differentiate these cell lines.
MATERIALS AND METHODS
METHODS
We utilized advanced imaging techniques such as holographic time-lapse microscopy, which provides real-time, three-dimensional imaging of cells, to conduct our comparative analysis. This allowed us to examine dynamic cellular morphology and behavior with exceptional sensitivity and resolution over time. The primary features assessed in our study included texture clustershade, area (μm
RESULTS
RESULTS
Our findings highlighted significant differences in the morphological features of MCF10A and MCF7 cells. MCF10A cells showed a higher texture clustershade value, suggesting less symmetry than MCF7 cells. On the other hand, MCF7 cells had smaller cellular area, higher eccentricity, lower irregularity, higher phase shift sum, higher optical volume, higher shape convexity, and higher hull convexity compared to MCF10A cells. These results suggest that MCF7 cells are smaller, more circular, less irregular, exhibit different light properties, and have a closer to perfect 3D shape relative to MCF10A cells.
CONCLUSION
CONCLUSIONS
The identified morphological differences between MCF10A and MCF7 cells offer valuable insights into the characteristics distinguishing normal breast cells from breast cancer cells. These findings not only contribute to our understanding of the morphological variability in breast cancer but also underscore the potential utility of these differences in future cancer diagnostics and treatment strategies.
Identifiants
pubmed: 37648303
pii: 43/9/3891
doi: 10.21873/anticanres.16576
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3891-3896Informations de copyright
Copyright © 2023 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.