Transcriptome of Oral Cancer Cells Adapted to Suspension Culture Is Potentially Related to Cancer Progressive Phenotypes.

Humans Transcriptome Mouth Neoplasms / genetics Gene Expression Profiling Immunologic Tests Apoptosis / genetics
Oral cancer cells anchorage-independent survival suspension culture transcriptome

Journal

Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988

Informations de publication

Date de publication:
Sep 2023
Historique:
received: 17 06 2023
revised: 25 07 2023
accepted: 27 07 2023
medline: 1 9 2023
pubmed: 31 8 2023
entrez: 30 8 2023
Statut: ppublish

Résumé

Cervical lymph node metastasis worsens oral cancer prognosis. Cancer cells with high metastatic ability can delay or resist apoptosis and survive in the floating condition during circulation. The involved genes and pathways in this process remain largely unknown. This study aimed to establish an oral cancer cell line adapted to suspension culture by in vitro selection and perform gene expression analysis. The oral cancer cell subline adapted to suspension culture was isolated by in vitro selection from the oral cancer cell line, HSC-3. The transcriptome profiles of HSC-3 and its subline were compared using gene expression microarrays. Gene Ontology (GO) enrichment analysis, Gene Set Enrichment Analysis (GSEA), and Ingenuity Pathway Analysis (IPA) were performed to predict the involved pathways and molecules in cancer progression. The subline was designated as HSC-3 Our transcriptome analysis revealed several potentially activated pathways and molecules in the floating-adapted oral cancer cells and indicated molecular implications for cancer progression.

Sections du résumé

BACKGROUND/AIM OBJECTIVE
Cervical lymph node metastasis worsens oral cancer prognosis. Cancer cells with high metastatic ability can delay or resist apoptosis and survive in the floating condition during circulation. The involved genes and pathways in this process remain largely unknown. This study aimed to establish an oral cancer cell line adapted to suspension culture by in vitro selection and perform gene expression analysis.
MATERIALS AND METHODS METHODS
The oral cancer cell subline adapted to suspension culture was isolated by in vitro selection from the oral cancer cell line, HSC-3. The transcriptome profiles of HSC-3 and its subline were compared using gene expression microarrays. Gene Ontology (GO) enrichment analysis, Gene Set Enrichment Analysis (GSEA), and Ingenuity Pathway Analysis (IPA) were performed to predict the involved pathways and molecules in cancer progression.
RESULTS RESULTS
The subline was designated as HSC-3
CONCLUSION CONCLUSIONS
Our transcriptome analysis revealed several potentially activated pathways and molecules in the floating-adapted oral cancer cells and indicated molecular implications for cancer progression.

Identifiants

DOI: 10.21873/anticanres.16578 PMID: 37648334
pubmed: 37648334
pii: 43/9/3905
doi: 10.21873/anticanres.16578
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

3905-3911

Informations de copyright

Copyright © 2023 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Auteurs

Kouhei Sakurai (K)

Department of Joint Research Laboratory of Clinical Medicine, School of Medicine, Fujita Health University, Aichi, Japan; kouhei.sakurai@fujita-u.ac.jp.

Tatsuya Ando (T)

Department of Joint Research Laboratory of Clinical Medicine, School of Medicine, Fujita Health University, Aichi, Japan.

Yuka Ideta (Y)

Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Yuichiro Hayashi (Y)

Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.
Department of Oral and Maxillofacial Surgery, Shonan Kamakura General Hospital, Kanagawa, Japan.

Junichi Baba (J)

Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.
Department of Oral and Maxillofacial Surgery, Saiseikai Yokohamashi Nanbu Hospital, Kanagawa, Japan.

Kenji Mitsudo (K)

Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Hiroyasu Ito (H)

Department of Joint Research Laboratory of Clinical Medicine, School of Medicine, Fujita Health University, Aichi, Japan.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Transcriptome of Oral Cancer Cells Adapted to...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Transcriptome › Transcriptome of Oral Cancer Cells Adapted to...
questionsmedicales.fr › Maladies du système stomatognathique › Maladies de la bouche › Tumeurs de la bouche › Transcriptome of Oral Cancer Cells Adapted to...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de profil d'expression de gènes › Transcriptome of Oral Cancer Cells Adapted to...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Tests immunologiques › Transcriptome of Oral Cancer Cells Adapted to...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › Transcriptome of Oral Cancer Cells Adapted to...