Structural basis for ion selectivity in potassium-selective channelrhodopsins.

HcKCR MD simulation channelrhodopsin cryo-EM electrophysiology microbial opsin optogenetics potassium channel spectroscopy structure-guided engineering

Journal

Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066

Informations de publication

Date de publication:
28 09 2023
Historique:
received: 30 09 2022
revised: 11 05 2023
accepted: 07 08 2023
medline: 2 10 2023
pubmed: 1 9 2023
entrez: 31 8 2023
Statut: ppublish

Résumé

KCR channelrhodopsins (K

Identifiants

pubmed: 37652010
pii: S0092-8674(23)00863-2
doi: 10.1016/j.cell.2023.08.009
pmc: PMC7615185
mid: EMS189037
pii:
doi:

Substances chimiques

Channelrhodopsins 0
Ion Channels 0
Potassium RWP5GA015D

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4325-4344.e26

Subventions

Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : Medical Research Council
ID : MC_UP_A025_1012
Pays : United Kingdom
Organisme : NIMH NIH HHS
ID : R01 MH075957
Pays : United States

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The KCR variants are described in pending patent application material; these tools and all methods, protocols, clones, and sequences are freely available to nonprofit institutions and investigators. K.D. is a member of the Cell Advisory Board, a co-founder and Scientific Advisory Board member of Stellaromics and Maplight Therapeutics, and a Scientific Advisory Board member of BrightMinds Biosciences.

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Auteurs

Seiya Tajima (S)

Komaba Institute for Science, The University of Tokyo, Meguro, Tokyo, Japan.

Yoon Seok Kim (YS)

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Masahiro Fukuda (M)

Komaba Institute for Science, The University of Tokyo, Meguro, Tokyo, Japan.

YoungJu Jo (Y)

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Peter Y Wang (PY)

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Joseph M Paggi (JM)

Department of Computer Science, Stanford University, Stanford, CA, USA.

Masatoshi Inoue (M)

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Eamon F X Byrne (EFX)

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Koichiro E Kishi (KE)

Komaba Institute for Science, The University of Tokyo, Meguro, Tokyo, Japan.

Seiwa Nakamura (S)

Komaba Institute for Science, The University of Tokyo, Meguro, Tokyo, Japan.

Charu Ramakrishnan (C)

CNC Program, Stanford University, Stanford, CA, USA.

Shunki Takaramoto (S)

The Institute for Solid State Physics, The University of Tokyo, Kashiwa, Japan.

Takashi Nagata (T)

The Institute for Solid State Physics, The University of Tokyo, Kashiwa, Japan.

Masae Konno (M)

The Institute for Solid State Physics, The University of Tokyo, Kashiwa, Japan; PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan.

Masahiro Sugiura (M)

Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-ku, Japan.

Kota Katayama (K)

Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-ku, Japan.

Toshiki E Matsui (TE)

Komaba Institute for Science, The University of Tokyo, Meguro, Tokyo, Japan.

Keitaro Yamashita (K)

MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK.

Suhyang Kim (S)

Komaba Institute for Science, The University of Tokyo, Meguro, Tokyo, Japan.

Hisako Ikeda (H)

Komaba Institute for Science, The University of Tokyo, Meguro, Tokyo, Japan.

Jaeah Kim (J)

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Hideki Kandori (H)

Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-ku, Japan; OptoBioTechnology Research Center, Nagoya Institute of Technology, Showa-ku, Japan.

Ron O Dror (RO)

Department of Computer Science, Stanford University, Stanford, CA, USA; Institute for Computational and Mathematical Engineering, Stanford University, Stanford, CA, USA.

Keiichi Inoue (K)

The Institute for Solid State Physics, The University of Tokyo, Kashiwa, Japan.

Karl Deisseroth (K)

Department of Bioengineering, Stanford University, Stanford, CA, USA; CNC Program, Stanford University, Stanford, CA, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA; Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA. Electronic address: deissero@stanford.edu.

Hideaki E Kato (HE)

Komaba Institute for Science, The University of Tokyo, Meguro, Tokyo, Japan; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo, Japan; FOREST, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan. Electronic address: hekato@bio.c.u-tokyo.ac.jp.

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