Biochemical analysis of Komagataella phaffii oxidative folding proposes novel regulatory mechanisms of disulfide bond formation in yeast.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
31 08 2023
31 08 2023
Historique:
received:
11
05
2023
accepted:
25
08
2023
medline:
4
9
2023
pubmed:
1
9
2023
entrez:
31
8
2023
Statut:
epublish
Résumé
Oxidative protein folding in the endoplasmic reticulum (ER) is driven mainly by protein disulfide isomerase PDI and oxidoreductin Ero1. Their activity is tightly regulated and interconnected with the unfolded protein response (UPR). The mechanisms of disulfide bond formation have mainly been studied in human or in the yeast Saccharomyces cerevisiae. Here we analyze the kinetics of disulfide bond formation in the non-conventional yeast Komagataella phaffii, a common host for the production of recombinant secretory proteins. Surprisingly, we found significant differences with both the human and S. cerevisiae systems. Specifically, we report an inactive disulfide linked complex formed by K. phaffii Ero1 and Pdi1, similarly to the human orthologs, but not described in yeast before. Furthermore, we show how the interaction between K. phaffii Pdi1 and Ero1 is unaffected by the introduction of unfolded substrate into the system. This is drastically opposed to the previously observed behavior of the human pathway, suggesting a different regulation of the UPR and/or possibly different interaction mechanics between K. phaffii Pdi1 and Ero1.
Identifiants
pubmed: 37652992
doi: 10.1038/s41598-023-41375-z
pii: 10.1038/s41598-023-41375-z
pmc: PMC10471769
doi:
Substances chimiques
Disulfides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14298Informations de copyright
© 2023. Springer Nature Limited.
Références
J Biol Chem. 2020 Jun 26;295(26):8647-8655
pubmed: 32102847
EMBO J. 1998 Feb 16;17(4):927-35
pubmed: 9463371
Cell. 2007 Apr 20;129(2):333-44
pubmed: 17448992
Cold Spring Harb Symp Quant Biol. 1987;52:511-9
pubmed: 2456884
Microb Cell Fact. 2017 Jun 15;16(1):108
pubmed: 28619018
Cell Rep. 2013 Apr 25;3(4):1279-92
pubmed: 23583182
J Cell Biol. 2013 Sep 16;202(6):861-74
pubmed: 24043701
Science. 2011 Nov 25;334(6059):1081-6
pubmed: 22116877
EMBO J. 2008 Nov 19;27(22):2988-97
pubmed: 18971943
Mol Biol Cell. 2006 May;17(5):2256-66
pubmed: 16495342
EMBO J. 2010 Oct 6;29(19):3330-43
pubmed: 20834232
Biochem J. 2014 Jul 1;461(1):107-13
pubmed: 24758166
Microb Cell Fact. 2021 Jun 30;20(1):124
pubmed: 34193127
Cell. 2004 May 28;117(5):601-10
pubmed: 15163408
Nat Protoc. 2007;2(1):35-7
pubmed: 17401335
J Cell Biol. 2002 Jul 22;158(2):247-57
pubmed: 12119363
Science. 1992 Sep 11;257(5076):1496-502
pubmed: 1523409
Microb Cell Fact. 2010 Sep 13;9:67
pubmed: 20836848
Nat Biotechnol. 2022 Dec;40(12):1722-1760
pubmed: 36471135
Microb Cell Fact. 2010 May 12;9:31
pubmed: 20462406
Life Sci Alliance. 2018 Jun 26;1(3):e201800090
pubmed: 30456358
Science. 2011 Nov 25;334(6059):1086-90
pubmed: 22116878
J Biol Chem. 2011 May 6;286(18):16261-71
pubmed: 21398518
Biotechnol Adv. 2023 May-Jun;64:108121
pubmed: 36775001
Antioxid Redox Signal. 2008 May;10(5):963-72
pubmed: 18205546
Trends Biochem Sci. 2018 Aug;43(8):593-605
pubmed: 30056836
Arch Biochem Biophys. 2013 Mar;531(1-2):100-9
pubmed: 23022410
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
J Biol Chem. 2014 Nov 7;289(45):31188-99
pubmed: 25258311
Cold Spring Harb Perspect Biol. 2019 May 1;11(5):
pubmed: 30396882
Nucleic Acids Res. 2022 Jan 7;50(D1):D439-D444
pubmed: 34791371
Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):299-304
pubmed: 16407158
N Biotechnol. 2023 Mar 25;73:19-28
pubmed: 36603701
BMC Syst Biol. 2017 Dec 08;11(1):123
pubmed: 29221460
FEBS Lett. 2001 Nov 9;508(1):117-20
pubmed: 11707280
J Mol Biol. 1995 Jun 2;249(2):463-77
pubmed: 7540214
Mol Cell. 1998 Jan;1(2):161-70
pubmed: 9659913
EMBO J. 2010 Oct 6;29(19):3318-29
pubmed: 20802462
Mol Cell. 1998 Jan;1(2):171-82
pubmed: 9659914
J Mol Biol. 1992 Oct 5;227(3):757-75
pubmed: 1383552
EMBO J. 2016 Apr 15;35(8):845-65
pubmed: 26869642
Nat Rev Mol Cell Biol. 2020 Aug;21(8):421-438
pubmed: 32457508
Trends Biotechnol. 2022 May;40(5):576-590
pubmed: 34924209
Antioxid Redox Signal. 2009 Nov;11(11):2807-50
pubmed: 19476414
Biochemistry. 1997 Mar 18;36(11):3383-8
pubmed: 9116018
Cell. 2018 Nov 29;175(6):1533-1545.e20
pubmed: 30415838
Antioxid Redox Signal. 2019 Aug 1;31(4):261-274
pubmed: 30880408
EMBO J. 2008 Nov 19;27(22):2977-87
pubmed: 18833192
Nat Rev Mol Cell Biol. 2003 Mar;4(3):181-91
pubmed: 12612637