Calcium-gated potassium channel blockade via membrane-facing fenestrations.


Journal

Nature chemical biology
ISSN: 1552-4469
Titre abrégé: Nat Chem Biol
Pays: United States
ID NLM: 101231976

Informations de publication

Date de publication:
31 Aug 2023
Historique:
received: 04 06 2021
accepted: 18 07 2023
medline: 1 9 2023
pubmed: 1 9 2023
entrez: 31 8 2023
Statut: aheadofprint

Résumé

Quaternary ammonium blockers were previously shown to bind in the pore to block both open and closed conformations of large-conductance calcium-activated potassium (BK and MthK) channels. Because blocker entry was assumed through the intracellular entryway (bundle crossing), closed-pore access suggested that the gate was not at the bundle crossing. Structures of closed MthK, a Methanobacterium thermoautotrophicum homolog of BK channels, revealed a tightly constricted intracellular gate, leading us to investigate the membrane-facing fenestrations as alternative pathways for blocker access directly from the membrane. Atomistic free energy simulations showed that intracellular blockers indeed access the pore through the fenestrations, and a mutant channel with narrower fenestrations displayed no closed-state TPeA block at concentrations that blocked the wild-type channel. Apo BK channels display similar fenestrations, suggesting that blockers may use them as access paths into closed channels. Thus, membrane fenestrations represent a non-canonical pathway for selective targeting of specific channel conformations, opening novel ways to selectively drug BK channels.

Identifiants

pubmed: 37653172
doi: 10.1038/s41589-023-01406-2
pii: 10.1038/s41589-023-01406-2
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)
ID : 088352
Organisme : Medical Advances Without Animals Trust (MAWA)
ID : N/A
Organisme : Medical Advances Without Animals Trust (MAWA)
ID : N/A
Organisme : Department of Education and Training | Australian Research Council (ARC)
ID : DP210102405
Organisme : Department of Education and Training | Australian Research Council (ARC)
ID : DP2201035501
Organisme : Department of Health | National Health and Medical Research Council (NHMRC)
ID : APP1141974

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.

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Auteurs

Chen Fan (C)

Department of Anesthesiology, Weill Cornell Medicine, New York, NY, USA.
Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden.

Emelie Flood (E)

School of Science, RMIT University, Melbourne, Victoria, Australia.
Schrödinger, Inc., New York, NY, USA.

Nattakan Sukomon (N)

Department of Anesthesiology, Weill Cornell Medicine, New York, NY, USA.

Shubhangi Agarwal (S)

Department of Anesthesiology, Weill Cornell Medicine, New York, NY, USA.

Toby W Allen (TW)

School of Science, RMIT University, Melbourne, Victoria, Australia. toby.allen@rmit.edu.au.

Crina M Nimigean (CM)

Department of Anesthesiology, Weill Cornell Medicine, New York, NY, USA. crn2002@med.cornell.edu.
Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA. crn2002@med.cornell.edu.

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