Retinoic acid and proteotoxic stress induce AML cell death overcoming stromal cell protection.

AML Actin cytoskeleton Bone marrow stromal cells ER stress Oxidative stress Proteotoxic stress Tumor microenvironment YAP

Journal

Journal of experimental & clinical cancer research : CR
ISSN: 1756-9966
Titre abrégé: J Exp Clin Cancer Res
Pays: England
ID NLM: 8308647

Informations de publication

Date de publication:
31 Aug 2023
Historique:
received: 16 05 2023
accepted: 10 08 2023
medline: 4 9 2023
pubmed: 1 9 2023
entrez: 31 8 2023
Statut: epublish

Résumé

Acute myeloid leukemia (AML) patients bearing the ITD mutation in the tyrosine kinase receptor FLT3 (FLT3-ITD) present a poor prognosis and a high risk of relapse. FLT3-ITD is retained in the endoplasmic reticulum (ER) and generates intrinsic proteotoxic stress. We devised a strategy based on proteotoxic stress, generated by the combination of low doses of the differentiating agent retinoic acid (R), the proteasome inhibitor bortezomib (B), and the oxidative stress inducer arsenic trioxide (A). We treated FLT3-ITD The combination RBA exerts strong cytotoxic activity on FLT3-ITD Our findings strengthen our previous work indicating induction of proteotoxic stress as a possible strategy in FLT3-ITD

Sections du résumé

BACKGROUND BACKGROUND
Acute myeloid leukemia (AML) patients bearing the ITD mutation in the tyrosine kinase receptor FLT3 (FLT3-ITD) present a poor prognosis and a high risk of relapse. FLT3-ITD is retained in the endoplasmic reticulum (ER) and generates intrinsic proteotoxic stress. We devised a strategy based on proteotoxic stress, generated by the combination of low doses of the differentiating agent retinoic acid (R), the proteasome inhibitor bortezomib (B), and the oxidative stress inducer arsenic trioxide (A).
METHODS METHODS
We treated FLT3-ITD
RESULTS RESULTS
The combination RBA exerts strong cytotoxic activity on FLT3-ITD
CONCLUSIONS CONCLUSIONS
Our findings strengthen our previous work indicating induction of proteotoxic stress as a possible strategy in FLT3-ITD

Identifiants

pubmed: 37653435
doi: 10.1186/s13046-023-02793-z
pii: 10.1186/s13046-023-02793-z
pmc: PMC10469880
doi:

Substances chimiques

Tretinoin 5688UTC01R
Ascorbic Acid PQ6CK8PD0R

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

223

Subventions

Organisme : Fondazione AIRC per la ricerca sul cancro ETS
ID : IG 2018-ID 21406

Informations de copyright

© 2023. Italian National Cancer Institute ‘Regina Elena’.

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Auteurs

Francesca Liccardo (F)

Department of Anatomical, Histological, Forensic Medicine and Orthopedic Sciences, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy.

Martyna Śniegocka (M)

Department of Anatomical, Histological, Forensic Medicine and Orthopedic Sciences, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy.

Claudia Tito (C)

Department of Anatomical, Histological, Forensic Medicine and Orthopedic Sciences, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy.

Alessia Iaiza (A)

Department of Anatomical, Histological, Forensic Medicine and Orthopedic Sciences, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy.

Tiziana Ottone (T)

Department of Biomedicine and Prevention, University of Tor Vergata, Rome, Italy.
Santa Lucia Foundation, I.R.C.C.S., Neuro-Oncohematology, Rome, Italy.

Mariadomenica Divona (M)

Department of Biomedicine and Prevention, University of Tor Vergata, Rome, Italy.

Serena Travaglini (S)

Department of Biomedicine and Prevention, University of Tor Vergata, Rome, Italy.

Maurizio Mattei (M)

Department of Biology, University of Tor Vergata, Rome, Italy.
Centro Interdipartimentale-CIMETA, University of Tor Vergata, Rome, Italy.

Rosella Cicconi (R)

Centro Interdipartimentale-CIMETA, University of Tor Vergata, Rome, Italy.

Selenia Miglietta (S)

Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Section of Human Anatomy, Sapienza University of Rome, Rome, Italy.

Giuseppe Familiari (G)

Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Section of Human Anatomy, Sapienza University of Rome, Rome, Italy.

Stefania Annarita Nottola (SA)

Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Section of Human Anatomy, Sapienza University of Rome, Rome, Italy.

Vincenzo Petrozza (V)

Department of Medico-Surgical Sciences & Biotechnologies, Center for Biophotonics, Sapienza University of Rome, Latina, Italy.

Luca Tamagnone (L)

Department of Life Sciences and Public Health, Histology and Embryology Unit, Catholic University of the Sacred Hearth, Rome, Italy.
Fondazione Policlinico Universitario "A. Gemelli", IRCCS, Rome, Italy.

Maria Teresa Voso (MT)

Department of Biomedicine and Prevention, University of Tor Vergata, Rome, Italy.
Santa Lucia Foundation, I.R.C.C.S., Neuro-Oncohematology, Rome, Italy.

Silvia Masciarelli (S)

Department of Anatomical, Histological, Forensic Medicine and Orthopedic Sciences, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy. silvia.masciarelli@uniroma1.it.

Francesco Fazi (F)

Department of Anatomical, Histological, Forensic Medicine and Orthopedic Sciences, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy. francesco.fazi@uniroma1.it.

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